Characterization of human gene expression changes after olive oil ingestion: an exploratory approach

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• dc.contributor.author Konstantinidou, Valentinica
• dc.contributor.author Khymenets, Olha, 1974-ca
• dc.contributor.author Fitó Colomer, Montserratca
• dc.contributor.author Torre Fornell, Rafael de laca
• dc.contributor.author Anglada Busquets, Rogerca
• dc.contributor.author Dopazo, Anaca
• dc.contributor.author Covas Planells, Maria Isabelca
• dc.date.accessioned 2012-04-17T12:23:30Z
• dc.date.available 2012-04-17T12:23:30Z
• dc.date.issued 2009ca
• dc.description.abstract Olive oil consumption is protective against risk factors for cardiovascular and cancer diseases. A nutrigenomic approach was performed to assess whether changes in gene expression could occur in human peripheral blood mononuclear cells after oli ve oil ingestion at postprandial state. Six healthy male volunteers ingested, at fasting state, 50 ml of olive oil. Prior to intervention a 1-week washout period with a controlled diet and sunflower oil as the only source of fat was followed. During the 3 days before and on the intervention day, a very low-phenolic compound diet was followed. At baseline (0 h) and at post-ingestion (6 h), total RNA was isolated and gene expression (29,082 genes) was evaluated by microarray. From microarray data, nutrient-gene interactions were observed in genes related to metabolism, cellular processes, cancer, and atherosclerosis (e.g. USP48 by 2.16; OGT by 1.68-fold change) and associated processes such as inflammation (e.g. AKAP13 by 2.30; IL-10 by 1.66-fold change) and DNA damage (e.g. DCLRE1C by 1.47; POLK by 1.44- fold change). When results obtained by microarray were verified by qRT-PCR in nine genes, full concordance was achieved only in the case of up-regulated genes. Changes were observed at a real-life dose of olive oil, as it is daily consumed in some Mediterranean areas. Our results support the hypothesis that postprandial protective changes related to olive oil consumption could be mediated through gene expression changes.
• dc.format.mimetype application/pdfca
• dc.identifier.citation Konstantinidou V, Khymenets O, Fitó M, Torre R, Anglada R, Dopazos A, Covas MI. Characterization of human gene expression changes after olive oil ingestion: an exploratory approach. Folia Biologica (Praha): 2009;55(3):85-91ca
• dc.identifier.issn 0015-5500ca
• dc.identifier.uri http://hdl.handle.net/10230/16370
• dc.language.iso engca
• dc.publisher Univerzita Karlova v Praze, Fakulta Socialnich Vedca
• dc.relation.ispartof Folia Biologica (Praha): 2009;55(3):85-91
• dc.rights © 2009 First Faculty of Medicine, Charles University, Prague, Czech/nRepublic. Published at Folia Biologica (Praha), vol. 55(3), p. 85-91, 2009ca
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.subject.keyword Olive oil
• dc.subject.keyword Gene expression
• dc.subject.keyword Microarray
• dc.subject.keyword Atherosclerosis
• dc.subject.keyword Cancer
• dc.subject.other Sistema cardiovascular -- Malalties -- Dietoteràpia
• dc.subject.other Nutrició -- Aspectes genètics
• dc.subject.other Oli d'oliva -- Ús terapèutic
• dc.title Characterization of human gene expression changes after olive oil ingestion: an exploratory approachca
• dc.type info:eu-repo/semantics/articleca
• dc.type.version info:eu-repo/semantics/publishedVersion