Oligonucleotide-lipid conjugates forming G-quadruplex structures are potent and pangenotypic hepatitis C virus entry inhibitors in vitro and ex vivo
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- dc.contributor.author Koutsoudakis, Georgeca
- dc.contributor.author Paris de León, Alexiaca
- dc.contributor.author Herrera, Carolinaca
- dc.contributor.author Dorner, Marcusca
- dc.contributor.author Pérez Vilaró, Gemma, 1985-ca
- dc.contributor.author Lyonnais, Sébastienca
- dc.contributor.author Grijalvo, Santiagoca
- dc.contributor.author Eritja, Ramonca
- dc.contributor.author Meyerhans, Andreasca
- dc.contributor.author Mirambeau, Gillesca
- dc.contributor.author Díez Antón, Juana, 1962-ca
- dc.date.accessioned 2017-06-22T07:28:30Z
- dc.date.available 2017-06-22T07:28:30Z
- dc.date.issued 2017
- dc.description.abstract A hepatitis C virus (HCV) epidemic affecting HIV-infected men who have sex with men (MSM) is expanding worldwide. In spite of the improved cure rates obtained with the new direct-acting antiviral drug (DAA) combinations, the high rate of reinfection within this population calls urgently for novel preventive interventions. In this study, we determined in cell culture and ex vivo experiments with human colorectal tissue that lipoquads, G-quadruplex DNA structures fused to cholesterol, are efficient HCV pangenotypic entry and cell-to-cell transmission inhibitors. Thus, lipoquads may be promising candidates for the development of rectally applied gels to prevent HCV transmission.
- dc.description.sponsorship The authors are supported by grants BFU2013-44629-R (to JD, GK and APL), and SAF2013-46077-R (to AM), and also through the “María de Maeztu” Programme for Units of Excellence in R&D (MDM-2014-0370), all from the Spanish Ministry of Economy and Competitiveness and FEDER. Work at Imperial College (CH) was funded by EC FP7 grant 305578 (PathCO).
- dc.format.mimetype application/pdfca
- dc.identifier.citation Koutsoudakis G, Paris de León A, Herrera C, Dorner M, Pérez Vilaró G, Lyonnais S et. Oligonucleotide-lipid conjugates forming G-quadruplex structures are potent and pangenotypic hepatitis C virus entry inhibitors in vitro and ex vivo. Antimicrobial Agents and Chemotherapy. 2017;61(5):e02354. DOI: 10.1128/AAC.02354-16
- dc.identifier.doi http://dx.doi.org/10.1128/AAC.02354-16
- dc.identifier.issn 0066-4804
- dc.identifier.uri http://hdl.handle.net/10230/32392
- dc.language.iso eng
- dc.publisher American Society for Microbiologyca
- dc.relation.ispartof Antimicrobial Agents and Chemotherapy. 2017;61(5):e02354
- dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/305578
- dc.rights © American Society for Microbiology
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.subject.keyword Oligonucleotide-cholesterol conjugates
- dc.subject.keyword DNA G-quadruplex structures
- dc.subject.keyword Antiviral treatment
- dc.subject.keyword HCV entry inhibitor
- dc.subject.keyword HCV cell-to-cell inhibitor
- dc.subject.keyword HCV pangenotypic inhibitor
- dc.title Oligonucleotide-lipid conjugates forming G-quadruplex structures are potent and pangenotypic hepatitis C virus entry inhibitors in vitro and ex vivoca
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/acceptedVersion