Electrical polarization enables integrative quality control during bacterial differentiation into spores

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• dc.contributor.author Sirec, Teja
• dc.contributor.author Benarroch, Jonatan M.
• dc.contributor.author Buffard, Pauline
• dc.contributor.author García Ojalvo, Jordi
• dc.contributor.author Asally, Munehiro
• dc.date.accessioned 2019-09-05T15:58:09Z
• dc.date.available 2019-09-05T15:58:09Z
• dc.date.issued 2019
• dc.description.abstract Quality control of offspring is important for the survival of cells. However, the mechanisms by which quality of offspring cells may be checked while running genetic programs of cellular differentiation remain unclear. Here we investigated quality control during sporulating in Bacillus subtilis by combining single-cell time-lapse microscopy, molecular biology, and mathematical modeling. Our results revealed that the quality control via premature germination is coupled with the electrical polarization of outer membranes of developing forespores. The forespores that accumulate fewer cations on their surface are more likely to be aborted. This charge accumulation enables the projection of multi-dimensional information about the external environment and morphological development of the forespore into one-dimensional information of cation accumulation. We thus present a paradigm of cellular regulation by bacterial electrical signaling. Moreover, based on the insight we gain, we propose an electrophysiology-based approach of reducing the yield and quality of Bacillus endospores.
• dc.description.sponsorship This study was supported by the start-up fund from University of Warwick, SLS Pump priming fund, and the Royal Society Research Grant to M.A., BBSRC/EPSRC grant to WISB (BB/M017982/1), and the Spanish Ministry of Economy and Competitiveness and FEDER (FIS2015-66503-C3-1-P) and the Maria de Maeztu Program for Units of Excellence (project MDM-2014-0370) to J.G.-O. J.M.B. acknowledges the funding by the MRC Doctoral Training Partnership (MR/N014294/1). P.B. was supported by a scholarship from the Region Auvergne-Rhone-Alpes and the Université Grenoble Alpes.
• dc.format.mimetype application/pdf
• dc.identifier.citation Sirec T, Benarroch JM, Buffard P, Garcia-Ojalvo J, Asally M. Electrical polarization enables integrative quality control during bacterial differentiation into spores. iScience. 2019;16:378-89. DOI: 10.1016/j.isci.2019.05.044
• dc.identifier.doi http://dx.doi.org/10.1016/j.isci.2019.05.044
• dc.identifier.issn 2589-0042
• dc.identifier.uri http://hdl.handle.net/10230/42247
• dc.language.iso eng
• dc.publisher Elsevier
• dc.relation.ispartof iScience. 2019;16:378-89
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/FIS2015-66503-C3-1-P
• dc.rights © 2019 The Author(s).This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/
• dc.subject.keyword Microbiology
• dc.subject.keyword Microbial Physiology
• dc.subject.keyword Bioinformatics
• dc.subject.keyword Mathematical Biosciences
• dc.title Electrical polarization enables integrative quality control during bacterial differentiation into spores
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion