Bradykinin, but not muscarinic, inhibition of M-current in rat sympathetic ganglion neurons involves phospholipase C-β4
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- dc.contributor.author Haley, Jane E.ca
- dc.contributor.author Abogadie, Fe C.ca
- dc.contributor.author Fernández-Fernández, José Manuel, 1967-ca
- dc.contributor.author Dayrell, Marizaca
- dc.contributor.author Vallis, Yvonneca
- dc.contributor.author Buckley, Noel J.ca
- dc.contributor.author Brown, David A.ca
- dc.date.accessioned 2012-07-05T06:54:17Z
- dc.date.available 2012-07-05T06:54:17Z
- dc.date.issued 2000ca
- dc.description.abstract Rat superior cervical ganglion (SCG) neurons express low-threshold noninactivating M-type potassium channels (I-K(M)), which can be inhibited by activation of M-1 muscarinic receptors (M-1 mAChR) and bradykinin (BK) B-2 receptors. Inhibition by the M1 mAChR agonist oxotremorine methiodide (Oxo-M) is mediated, at least in part, by the pertussis toxin-insensitive G-protein G alpha (q) (Caulfield et al., 1994; Haley et al., 1998a), whereas BK inhibition involves G alpha (q) and/or G alpha (11) (Jones et al., 1995). G alpha (q) and G alpha (11) can stimulate phospholipase C-beta (PLC-beta), raising the possibility that PLC is involved in I-K(M) inhibition by Oxo-M and BK. RT-PCR and antibody staining confirmed the presence of PLC-beta1, - beta2, - beta3, and - beta4 in rat SCG. We have tested the role of two PLC isoforms (PLC-beta1 and PLC-beta4) using antisense-expression constructs. Antisense constructs, consisting of the cytomegalovirus promoter driving antisense cRNA corresponding to the 3'-untranslated regions of PLC-beta1 and PLC-beta4, were injected into the nucleus of dissociated SCG neurons. Injected cells showed reduced antibody staining for the relevant PLC-beta isoform when compared to uninjected cells 48 hr later. BK inhibition of I-K(M) was significantly reduced 48 hr after injection of the PLC-beta4, but not the PLC-beta1, antisense-encoding plasmid. Neither PLC-beta antisense altered M-1 mAChR inhibition by Oxo-M. These data support the conclusion of Cruzblanca et al. (1998) that BK, but not M-1 mAChR, inhibition of I-K(M) involves PLC and extends this finding by indicating that PLC-beta4 is involved.
- dc.format.mimetype application/pdfca
- dc.identifier.citation Haley JE, Abogadie FC, Fernandez-Fernandez JM, Dayrell M, Vallis Y, Buckley NJ et al. Bradykinin, but not muscarinic, inhibition of M-current in rat sympathetic ganglion neurons involves phospholipase C-beta 4. J Neurosci. 2000;20(21):RC105. DOI: 10.1523/jneurosci.20-21-j0003.2000ca
- dc.identifier.doi http://dx.doi.org/10.1523/jneurosci.20-21-j0003.2000
- dc.identifier.issn 0270-6474ca
- dc.identifier.uri http://hdl.handle.net/10230/16669
- dc.language.iso engca
- dc.publisher Society for Neuroscienceca
- dc.relation.ispartof Journal of Neuroscience. 2000;20(21):RC105
- dc.rights © 2000, Society for Neuroscience. The published version is available at: http://www.jneurosci.org/cgi/reprintframed/20/21/RC105ca
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.subject.keyword M-current
- dc.subject.keyword Muscarinic receptor
- dc.subject.keyword Bradykinin
- dc.subject.keyword Phospholipase C-b
- dc.subject.keyword Antisense
- dc.subject.keyword Superior cervical ganglion neuron
- dc.subject.other Xarxes neuronals (Neurobiologia)
- dc.subject.other Farmacologia molecular
- dc.title Bradykinin, but not muscarinic, inhibition of M-current in rat sympathetic ganglion neurons involves phospholipase C-β4ca
- dc.type info:eu-repo/semantics/articleca
- dc.type.version info:eu-repo/semantics/publishedVersion