Self-amplifying pulsatile protein dynamics without positive feedback

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• dc.contributor.author Martínez Corral, Rosa, 1991-
• dc.contributor.author Raimundez, Elba
• dc.contributor.author Lin, Yihan
• dc.contributor.author Elowitz, Michael B.
• dc.contributor.author García Ojalvo, Jordi
• dc.date.accessioned 2019-07-09T07:29:14Z
• dc.date.issued 2018
• dc.description.abstract Many proteins exhibit dynamic activation patterns in the form of irregular pulses. Such behavior is typically attributed to a combination of positive and negative feedback loops in the underlying regulatory network. However, the presence of positive feedbacks is difficult to demonstrate unequivocally, raising the question of whether stochastic pulses can arise from negative feedback only. Here, we use the protein kinase A (PKA) system, a key regulator of the yeast pulsatile transcription factor Msn2, as a case example to show that irregular pulses of protein activity can arise from a negative feedback loop alone. Simplification to two variables reveals that a combination of zero-order ultrasensitivity, timescale separation between the activator and the repressor, and an effective delay in the feedback are sufficient to amplify a perturbation into a pulse. The same circuit topology can account for both activation and inactivation pulses, pointing toward a general mechanism of stochastic pulse generation.
• dc.description.sponsorship This work was supported by the Spanish Ministry of Economy and Competitiveness and FEDER (project FIS2015-66503-C3-1-P), and by the Generalitat de Catalunya (project 2017SGR1054). R.M.C. acknowledges financial support from La Caixa foundation. J.G.O. acknowledges support from the ICREA Academia programme and from the “Mar ́ıa de Maeztu” Programme for Units of Excellence in R&D (Spanish Ministry of Economy and Competitiveness, MDM-2014-0370). Y.L. acknowledges the support from National Natural Science Foundation of China (Grant No. 31771425) and the Thousand Young Talents Program of China. M.B.E. is a Howard Hughes Medical Institute Investigator. This work was supported by National Science Foundation grant #1547056.
• dc.format.mimetype application/pdf
• dc.identifier.citation Martinez-Corral R, Raimundez E, Lin Y, Elowitz MB, Garcia-Ojalvo J. Self-amplifying pulsatile protein dynamics without positive feedback. Cell Syst. 2018;7(4):453-62. DOI: 10.1016/j.cels.2018.08.012
• dc.identifier.doi http://dx.doi.org/10.1016/j.cels.2018.08.012
• dc.identifier.issn 2405-4712
• dc.identifier.uri http://hdl.handle.net/10230/41962
• dc.language.iso eng
• dc.publisher Elsevier
• dc.relation.ispartof Cell Systems. 2018;7(4):453-62
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/FIS2015-66503-C3-1-P
• dc.rights © Elsevier http://dx.doi.org/10.1016/j.cels.2018.08.012
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.subject.keyword Biological noise
• dc.subject.keyword Cell signaling dynamics
• dc.subject.keyword Cellular oscillations
• dc.subject.keyword Feedback
• dc.subject.keyword Protein activation
• dc.subject.keyword Protein activity pulses
• dc.subject.keyword Ultrasensitivity
• dc.title Self-amplifying pulsatile protein dynamics without positive feedback
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/acceptedVersion