Circulating microRNA profiling is altered in the acute respiratory distress syndrome related to SARS-CoV-2 infection

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  • dc.contributor.author Garcia Giralt, Natàlia
  • dc.contributor.author Du, Juan
  • dc.contributor.author Marin Corral, Judith
  • dc.contributor.author Bódalo-Torruella, Marta
  • dc.contributor.author Blasco-Hernando, Fabiola
  • dc.contributor.author Muñoz-Bermúdez, Rosana
  • dc.contributor.author Clarós, Miquel
  • dc.contributor.author Nonell Mazelon, Lara, 1972-
  • dc.contributor.author Perera Bel, Júlia
  • dc.contributor.author Fernández-González, Marta
  • dc.contributor.author Nogués Solan, Francesc Xavier
  • dc.contributor.author Sorli Redó, M. Luisa
  • dc.contributor.author Güerri Fernández, Roberto
  • dc.date.accessioned 2022-12-09T08:05:10Z
  • dc.date.available 2022-12-09T08:05:10Z
  • dc.date.issued 2022
  • dc.description.abstract One of the hallmarks of SARS-CoV-2 infection is an induced immune dysregulation, in some cases resulting in cytokine storm syndrome and acute respiratory distress syndrome (ARDS). Several physiological parameters are altered as a result of infection and cytokine storm. Among them, microRNAs (miRNAs) might reflect this poor condition since they play a significant role in immune cellular performance including inflammatory responses. Circulating miRNAs in patients who underwent ARDS and needed mechanical ventilation (MV+; n = 15) were analyzed by next generation sequencing in comparison with patients who had COVID-19 poor symptoms but without intensive care unit requirement (MV-; n = 13). A comprehensive in silico analysis by integration with public gene expression dataset and pathway enrichment was performed. Whole miRNA sequencing identified 170 differentially expressed miRNAs between patient groups. After the validation step by qPCR in an independent sample set (MV+ = 10 vs. MV- = 10), the miR-369-3p was found significantly decreased in MV+ patients (Fold change - 2.7). After integrating with gene expression results from COVID-19 patients, the most significant GO enriched pathways were acute inflammatory response, regulation of transmembrane receptor protein Ser/Thr, fat cell differentiation, and regulation of biomineralization and ossification. In conclusion, miR-369-3p was altered in patients with mechanical ventilation requirement in comparison with COVID-19 patients without this requirement. This miRNA is involved in inflammatory response which it can be considered as a prognosis factor for ARDS in COVID-19 patients.
  • dc.format.mimetype application/pdf
  • dc.identifier.citation Garcia-Giralt N, Du J, Marin-Corral J, Bódalo-Torruella M, Blasco-Hernando F, Muñoz-Bermúdez R, et al. Circulating microRNA profiling is altered in the acute respiratory distress syndrome related to SARS-CoV-2 infection. Sci Rep. 2022 Apr 28; 12(1): 6929. DOI: 10.1038/s41598-022-10738-3
  • dc.identifier.doi http://dx.doi.org/10.1038/s41598-022-10738-3
  • dc.identifier.issn 2045-2322
  • dc.identifier.uri http://hdl.handle.net/10230/55095
  • dc.language.iso eng
  • dc.publisher Nature Research
  • dc.rights Copyright © The Author(s) 2022. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.rights.uri http://creativecommons.org/licenses/by/4.0/
  • dc.subject.other COVID-19 (Malaltia)
  • dc.subject.other MicroARN
  • dc.subject.other Citocines
  • dc.subject.other Aparell respiratori--Malalties
  • dc.title Circulating microRNA profiling is altered in the acute respiratory distress syndrome related to SARS-CoV-2 infection
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/publishedVersion

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Articles (Hospital del Mar Research Institute)
Articles (Departament de Medicina i Ciències de la Vida)