Nucleosome architecture throughout the cell cycle

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• dc.contributor.author Deniz, Özgenca
• dc.contributor.author Flores, Oscarca
• dc.contributor.author Aldea, Martíca
• dc.contributor.author Soler López, Montserratca
• dc.contributor.author Orozco, Modestoca
• dc.date.accessioned 2016-06-02T17:04:03Z
• dc.date.available 2016-06-02T17:04:03Z
• dc.date.issued 2016
• dc.description.abstract Nucleosomes provide additional regulatory mechanisms to transcription and DNA replication by mediating the access of proteins to DNA. During the cell cycle chromatin undergoes several conformational changes, however the functional significance of these changes to cellular processes are largely unexplored. Here, we present the first comprehensive genome-wide study of nucleosome plasticity at single base-pair resolution along the cell cycle in Saccharomyces cerevisiae. We determined nucleosome organization with a specific focus on two regulatory regions: transcription start sites (TSSs) and replication origins (ORIs). During the cell cycle, nucleosomes around TSSs display rearrangements in a cyclic manner. In contrast to gap (G1 and G2) phases, nucleosomes have a fuzzier organization during S and M phases, Moreover, the choreography of nucleosome rearrangements correlate with changes in gene expression during the cell cycle, indicating a strong association between nucleosomes and cell cycle-dependent gene functionality. On the other hand, nucleosomes are more dynamic around ORIs along the cell cycle, albeit with tighter regulation in early firing origins, implying the functional role of nucleosomes on replication origins. Our study provides a dynamic picture of nucleosome organization throughout the cell cycle and highlights the subsequent impact on transcription and replication activity.ca
• dc.description.sponsorship This work was supported by the Spanish Ministry of Science and Innovation (BIO2012-32868), Instituto de Salud Carlos III (INB) and the European Research Council (SimDNA project).
• dc.format.mimetype application/pdfca
• dc.identifier.citation Deniz Ö, Flores O, Aldea M, Soler-López M, Orozco M. Nucleosome architecture throughout the cell cycle. Scientific Reports. 2016; 6: 19729. DOI 10.1038/srep19729ca
• dc.identifier.doi http://dx.doi.org/10.1038/srep19729
• dc.identifier.issn 2041-1723
• dc.identifier.uri http://hdl.handle.net/10230/26809
• dc.language.iso engca
• dc.publisher Nature Publishing Groupca
• dc.relation.ispartof Scientific Reports. 2016; 6: 19729
• dc.rights © Nature Publishing Group. http://www.nature.com/articles/srep19729/nThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material.ca
• dc.rights.accessRights info:eu-repo/semantics/openAccessca
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/ca
• dc.subject.keyword Computational biology and bioinformatics
• dc.subject.keyword Nucleosomes
• dc.subject.other Biologia computacionalca
• dc.title Nucleosome architecture throughout the cell cycleca
• dc.type info:eu-repo/semantics/articleca
• dc.type.version info:eu-repo/semantics/publishedVersionca