Relationship between the respiratory microbiome and the severity of airflow limitation, history of exacerbations and circulating eosinophils in COPD patients

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• dc.contributor.author Millares, Laura
• dc.contributor.author Pascual Guàrdia, Sergi, 1979-
• dc.contributor.author Montón, Concepción
• dc.contributor.author García-Nuñez, Marian
• dc.contributor.author Lalmolda, Cristina
• dc.contributor.author Faner, Rosa
• dc.contributor.author Casadevall Fusté, Carme
• dc.contributor.author Setó, Laia
• dc.contributor.author Capilla, Silvia
• dc.contributor.author Moreno, Amàlia
• dc.contributor.author Castro-Acosta, Ady Angélica
• dc.contributor.author Alvarez-Martinez, Carlos José
• dc.contributor.author Sibila, Oriol
• dc.contributor.author Peces-Barba Romero, Germán
• dc.contributor.author Cosio, Borja G.
• dc.contributor.author Agustí García-Navarro, Àlvar
• dc.contributor.author Gea Guiral, Joaquim
• dc.contributor.author Monsó Molas, Eduard
• dc.date.accessioned 2019-09-06T14:15:38Z
• dc.date.available 2019-09-06T14:15:38Z
• dc.date.issued 2019
• dc.description.abstract Background: The respiratory microbiome is altered in COPD patients but its relationship with core components of the disease, such as the severity of airflow limitation, the frequency of exacerbations or the circulating levels of eosinophils, is unclear. Methods: Cross-sectional study comprising 72 clinically stable COPD patients (mean age 68 [SD 7.9] years; FEV1 48.7 [SD 20.1]% of reference) who provided spontaneous sputum samples for 16S rRNA gene amplification and sequencing. The microbiome composition was analysed with QIIME. Results: We observed that: (1) more severe airflow limitation was associated with reduced relative abundance (RA) of Treponema and an increase in Pseudomonas; (2) patients with ≥2 exacerbations the previous year showed a significantly different bacterial community with respect to non-exacerbators (p = 0.014), with changes in 13 genera, including an increase of Pseudomonas, and finally, (3) peripheral eosinophils levels ≥2% were associated with more diverse microbiome [Chao1 224.51 (74.88) vs 277.39 (78.92) p = 0.006; Shannon 3.94 (1.05) vs 4.54 (1.06) p = 0.020], and a significant increase in the RAs of 20 genera. Conclusion: The respiratory microbiome in clinically stable COPD patients varies significantly according to the severity of airflow limitation, previous history of exacerbations and circulating eosinophils levels.
• dc.description.sponsorship This study was funded by FIS PI15/00167 and PI15/02042 from Instituto de Salud Carlos III (ISCIII), SAF 2014–54371 (FEDER) from Ministerio de Economía y Competitividad, Sociedad Española de Neumología y Cirugía Torácica (SEPAR), Laboratorios Menarini S. A, Fundació Pla Armengol and Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES). CIBERES is an initiative of the ISCIII.
• dc.format.mimetype application/pdf
• dc.identifier.citation Millares L, Pascual S, Montón C, García-Núñez M, Lalmolda C, Faner R et al. Relationship between the respiratory microbiome and the severity of airflow limitation, history of exacerbations and circulating eosinophils in COPD patients. BMC Pulm Med. 2019;19(1):112. DOI: 10.1186/s12890-019-0867-x
• dc.identifier.doi http://dx.doi.org/10.1186/s12890-019-0867-x
• dc.identifier.issn 1471-2466
• dc.identifier.uri http://hdl.handle.net/10230/42250
• dc.language.iso eng
• dc.publisher BioMed Central
• dc.relation.ispartof BMC Pulmonary Medicine. 2019;19(1):112
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/SAF2014-54371
• dc.rights © The Author(s). 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Bacterial community
• dc.subject.keyword Diversity
• dc.subject.keyword Eosinophils
• dc.subject.keyword Exacerbations
• dc.subject.keyword Sputum
• dc.subject.keyword Stable COPD
• dc.title Relationship between the respiratory microbiome and the severity of airflow limitation, history of exacerbations and circulating eosinophils in COPD patients
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion