Collecting mouse livers for transcriptome analysis of daily rhythms

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  • dc.contributor.author Mortimer, Thomas
  • dc.contributor.author Welz, Patrick-Simon
  • dc.contributor.author Aznar Benitah, Salvador
  • dc.contributor.author Koronowski, Kevin B.
  • dc.date.accessioned 2021-11-04T07:36:50Z
  • dc.date.available 2021-11-04T07:36:50Z
  • dc.date.issued 2021
  • dc.description.abstract Molecular daily rhythms can be captured by precisely timed tissue harvests from groups of animals. This protocol will allow the investigator to identify transcriptional rhythms in the mouse liver while also providing a template for similar analyses in other whole metabolic organs. We describe steps for mouse entrainment, liver dissection, and rhythmicity analysis from total RNA sequencing data. The resulting rhythmic transcriptome will provide the user with a starting point for defining specific biological processes that undergo daily rhythms. For complete details on the use and execution of this protocol, please refer to Koronowski et al. (2019). A similar protocol for interfollicular epidermal cells is demonstrated in Welz et al. (2019).
  • dc.description.sponsorship The IRB Barcelona is a Severo Ochoa Center of Excellence (MINECO award SEV-2015-0505). K.B.K. was supported by NIH-NIDDK (award F32DK121425). T.M. received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No. 754510.
  • dc.format.mimetype application/pdf
  • dc.identifier.citation Mortimer T, Welz PS, Benitah SA, Koronowski KB. Collecting mouse livers for transcriptome analysis of daily rhythms. STAR Protoc. 2021;2(2):100539. DOI: 10.1016/j.xpro.2021.100539
  • dc.identifier.doi http://dx.doi.org/10.1016/j.xpro.2021.100539
  • dc.identifier.issn 2666-1667
  • dc.identifier.uri http://hdl.handle.net/10230/48894
  • dc.language.iso eng
  • dc.publisher Elsevier
  • dc.relation.ispartof STAR Protoc. 2021;2(2):100539
  • dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/754510
  • dc.rights © 2021 The Author(s).This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.rights.uri http://creativecommons.org/licenses/by/4.0/
  • dc.subject.keyword Cancer
  • dc.subject.keyword Metabolism
  • dc.subject.keyword RNA-seq
  • dc.title Collecting mouse livers for transcriptome analysis of daily rhythms
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/publishedVersion

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