The pluripotency state of human embryonic stem cells derived from single blastomeres of eight-cell embryos
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- dc.contributor.author Massafret, Ot
- dc.contributor.author Barragán, Montserrat
- dc.contributor.author Álvarez-González, Lucía
- dc.contributor.author Aran, Begoña
- dc.contributor.author Martín-Mur, Beatriz
- dc.contributor.author Esteve-Codina, Anna
- dc.contributor.author Ruiz Herrera, Aurora
- dc.contributor.author Ibáñez, Elena
- dc.contributor.author Santaló, Josep
- dc.date.accessioned 2024-09-23T06:32:49Z
- dc.date.available 2024-09-23T06:32:49Z
- dc.date.issued 2024
- dc.description.abstract Human embryonic stem cells (hESCs) derived from blastocyst stage embryos present a primed state of pluripotency, whereas mouse ESCs (mESCs) display naïve pluripotency. Their unique characteristics make naïve hESCs more suitable for particular applications in biomedical research. This work aimed to derive hESCs from single blastomeres and determine their pluripotency state, which is currently unclear. We derived hESC lines from single blastomeres of 8-cell embryos and from whole blastocysts, and analysed several naïve pluripotency indicators, their transcriptomic profile and their trilineage differentiation potential. No significant differences were observed between blastomere-derived hESCs (bm-hESCs) and blastocyst-derived hESCs (bc-hESCs) for most naïve pluripotency indicators, including TFE3 localization, mitochondrial activity, and global DNA methylation and hydroxymethylation, nor for their trilineage differentiation potential. Nevertheless, bm-hESCs showed an increased single-cell clonogenicity and a higher expression of naïve pluripotency markers at early passages than bc-hESCs. Furthermore, RNA-seq revealed that bc-hESCs overexpressed a set of genes related to the post-implantational epiblast. Altogether, these results suggest that bm-hESCs, although displaying primed pluripotency, would be slightly closer to the naïve end of the pluripotency continuum than bc-hESCs.
- dc.description.sponsorship This work was supported by the Department of Research and Universities of the Generalitat de Catalunya (2021 SGR 00122) and the Spanish Ministry of Science and Innovation (PID2020-112557GB-I00 to A.R.-H.). O.M. was beneficiary of a FI-2017 fellowship by Generalitat de Catalunya. L.A.-G. was supported by a FPI predoctoral fellowship from the Spanish Ministry of Economy and Competitiveness (PRE-2018-083257).
- dc.format.mimetype application/pdf
- dc.identifier.citation Massafret O, Barragán M, Álvarez-González L, Aran B, Martín-Mur B, Esteve-Codina A, et al. The pluripotency state of human embryonic stem cells derived from single blastomeres of eight-cell embryos. Cells Dev. 2024 Sep;179:203935. DOI: 10.1016/j.cdev.2024.203935
- dc.identifier.doi http://dx.doi.org/10.1016/j.cdev.2024.203935
- dc.identifier.issn 2667-291X
- dc.identifier.uri http://hdl.handle.net/10230/61199
- dc.language.iso eng
- dc.publisher Elsevier
- dc.relation.ispartof Cells Dev. 2024 Sep;179:203935
- dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PID2020-112557GB-I00
- dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PRE-2018-083257
- dc.rights © 2024 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri http://creativecommons.org/licenses/by/4.0/
- dc.subject.keyword Blastomeres
- dc.subject.keyword Derivation
- dc.subject.keyword Naïve
- dc.subject.keyword Pluripotency
- dc.subject.keyword Primed
- dc.subject.keyword hESC
- dc.title The pluripotency state of human embryonic stem cells derived from single blastomeres of eight-cell embryos
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion