Effect of mutation order on myeloproliferative neoplasms.

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• dc.contributor.author Ortmann, Christina A.ca
• dc.contributor.author Green, Anthony R.ca
• dc.contributor.author Bellosillo Paricio, Beatrizca
• dc.contributor.author Besses Raebel, Carlesca
• dc.date.accessioned 2015-06-15T11:26:30Z
• dc.date.available 2015-08-31T02:00:03Z
• dc.date.issued 2015
• dc.description.abstract BACKGROUND: Cancers result from the accumulation of somatic mutations, and their properties are thought to reflect the sum of these mutations. However, little is known about the effect of the order in which mutations are acquired. METHODS:/nWe determined mutation order in patients with myeloproliferative neoplasms by genotyping hematopoietic colonies or by means of next-generation sequencing. Stem cells and progenitor cells were isolated to study the effect of mutation order on mature and immature hematopoietic cells. RESULTS: The age at which a patient presented with a myeloproliferative neoplasm, acquisition of JAK2 V617F homozygosity, and the balance of immature progenitors were all influenced by mutation order. As compared with patients in whom the TET2 mutation was acquired first (hereafter referred to as "TET2-first patients"), patients in whom the Janus kinase 2 (JAK2) mutation was acquired first ("JAK2-first patients") had a greater likelihood of presenting with polycythemia vera than with essential thrombocythemia, an increased risk of thrombosis, and an increased sensitivity of JAK2-mutant progenitors to ruxolitinib in vitro. Mutation order influenced the proliferative response to JAK2 V617F and the capacity of double-mutant hematopoietic cells and progenitor cells to generate colony-forming cells. Moreover, the hematopoietic stem-and-progenitor-cell compartment was dominated by TET2 single-mutant cells in TET2-first patients but by JAK2-TET2 double-mutant cells in JAK2-first patients. Prior mutation of TET2 altered the transcriptional consequences of JAK2 V617F in a cell-intrinsic manner and prevented JAK2 V617F from up-regulating genes associated with proliferation. CONCLUSIONS: The order in which JAK2 and TET2 mutations were acquired influenced clinical features, the response to targeted therapy, the biology of stem and progenitor cells, and clonal evolution in patients with myeloproliferative neoplasms. (Funded by Leukemia and Lymphoma Research and others.).ca
• dc.description.sponsorship Work in the Green lab is supported by Leukemia and Lymphoma Research, Cancer Research UK, the Kay Kendall Leukaemia Fund, the NIHR Cambridge Biomedical Research Centre, the Cambridge Experimental Cancer Medicine Centre, and the Leukemia & Lymphoma Society of America. DGK was supported by a postdoctoral fellowship from the Canadian Institutes of Health Research (Ottawa, ON), and a Lady Tata Memorial Trust International Award for Research in Leukaemia (London, UK). CAO was supported by a research fellowship from the Deutsche Forschungsgemeinschaft (DFG, OR255/1-1).
• dc.format.mimetype application/pdfca
• dc.identifier.citation Ortmann CA, Kent DG, Nangalia J, Silber Y, Wedge DC, Grinfeld J. et al. Effect of mutation order on myeloproliferative neoplasms. N Engl J Med. 2015 Feb 12;372(7):601-12. doi: 10.1056/NEJMoa1412098.ca
• dc.identifier.doi http://dx.doi.org/10.1056/NEJMoa1412098
• dc.identifier.issn 0028-4793
• dc.identifier.uri http://hdl.handle.net/10230/23823
• dc.language.iso engca
• dc.publisher Massachusets medical societyca
• dc.relation.ispartof The New England Journal of Medicine. 2015 Feb 12;372(7):601-12
• dc.rights From New England Journal of Medicine, Ortmann CA, Kent DG, Nangalia J, Silber Y, Wedge DC, Grinfeld J. et al, Effect of mutation order on myeloproliferative neoplasms. 12;372(7):601-12. Copyright © 2015 Massachusetts Medical Society. Reprinted with permissionca
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.subject.other Càncerca
• dc.subject.other Tumorsca
• dc.title Effect of mutation order on myeloproliferative neoplasms.ca
• dc.type info:eu-repo/semantics/articleca
• dc.type.version info:eu-repo/semantics/acceptedVersionca