Comparing mitochondrial activity, oxidative stress tolerance, and longevity of thirteen Ascomycota yeast species

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• dc.contributor.author Gröger, Anna
• dc.contributor.author Martínez-Albo, Ilune
• dc.contributor.author Albà Soler, Mar
• dc.contributor.author Ayté del Olmo, José
• dc.contributor.author Vega, Montserrat
• dc.contributor.author Hidalgo Hernando, Elena
• dc.date.accessioned 2023-12-12T06:57:58Z
• dc.date.available 2023-12-12T06:57:58Z
• dc.date.issued 2023
• dc.description.abstract Aging is characterized by a number of hallmarks including loss of mitochondrial homeostasis and decay in stress tolerance, among others. Unicellular eukaryotes have been widely used to study chronological aging. As a general trait, calorie restriction and activation of mitochondrial respiration has been proposed to contribute to an elongated lifespan. Most aging-related studies have been conducted with the Crabtree-positive yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe, and with deletion collections deriving from these conventional yeast models. We have performed an unbiased characterization of longevity using thirteen fungi species, including S. cerevisiae and S. pombe, covering a wide range of the Ascomycota clade. We have determined their mitochondrial activity by oxygen consumption, complex IV activity, and mitochondrial redox potential, and the results derived from these three methodologies are highly overlapping. We have phenotypically compared the lifespans of the thirteen species and their capacity to tolerate oxidative stress. Longevity and elevated tolerance to hydrogen peroxide are correlated in some but not all yeasts. Mitochondrial activity per se cannot anticipate the length of the lifespan. We have classified the strains in four groups, with members of group 1 (Kluyveromyces lactis, Saccharomyces bayanus and Lodderomyces elongisporus) displaying high mitochondrial activity, elevated resistance to oxidative stress, and elongated lifespan.
• dc.description.sponsorship This work is supported by grants PGC2018-093920-B-I00 and PID2021-122837NB-I00 funded by MICIN/AEI/10.13039/501100011033/FEDER, UE to E.H., funded by Ministerio de Ciencia e Innovación, Agencia Estatal de Investigación and Fondo Europeo de Desarrollo Regional. The Oxidative Stress and Cell Cycle group is also supported by Generalitat de Catalunya (Spain) (2017-SGR-539 and 2021-SGR-00007) and by Excellence Unit María de Maeztu Grant CEX2018-000792-M funded by MCIN/AEI/10.13039/501100011033. E.H. is recipient of an ICREA Academia Award (Generalitat de Catalunya, Spain).
• dc.format.mimetype application/pdf
• dc.identifier.citation Gröger A, Martínez-Albo I, Albà MM, Ayté J, Vega M, Hidalgo E. Comparing mitochondrial activity, oxidative stress tolerance, and longevity of thirteen Ascomycota yeast species. Antioxidants (Basel). 2023 Sep 28;12(10):1810. DOI: 10.3390/antiox12101810
• dc.identifier.doi http://dx.doi.org/10.3390/antiox12101810
• dc.identifier.issn 2076-3921
• dc.identifier.uri http://hdl.handle.net/10230/58515
• dc.language.iso eng
• dc.publisher MDPI
• dc.relation.ispartof Antioxidants (Basel). 2023 Sep 28;12(10):1810
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PGC2018-093920-B-I00
• dc.relation.projectID info:eu-repo/grantAgreement/ES/3PE/PID2021-122837NB-I00
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/CEX2018-000792-M
• dc.rights © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Ascomycota
• dc.subject.keyword H2O2 tolerance
• dc.subject.keyword S. cerevisiae
• dc.subject.keyword S. pombe
• dc.subject.keyword Aging
• dc.subject.keyword Mitochondrial activity
• dc.subject.keyword Respiration
• dc.title Comparing mitochondrial activity, oxidative stress tolerance, and longevity of thirteen Ascomycota yeast species
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion