The mechanosensitive Piezo1 channel controls endosome trafficking for an efficient cytokinetic abscission

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• dc.contributor.author Carrillo García, Julia, 1993-
• dc.contributor.author Herrera-Fernández, Víctor
• dc.contributor.author Serra, Selma A.
• dc.contributor.author Rubio Moscardó, Fanny
• dc.contributor.author Vogel-González, Marina
• dc.contributor.author Doñate-Macián, Pau
• dc.contributor.author Hevia, Covadonga F.
• dc.contributor.author Pujades Corbi, Cristina
• dc.contributor.author Valverde, M. A. (Miguel Ángel), 1963-
• dc.date.accessioned 2021-12-16T06:56:30Z
• dc.date.available 2021-12-16T06:56:30Z
• dc.date.issued 2021
• dc.description.abstract Mechanical forces are exerted throughout cytokinesis, the final step of cell division. Yet, how forces are transduced and affect the signaling dynamics of cytokinetic proteins remains poorly characterized. We now show that the mechanosensitive Piezo1 channel is activated at the intercellular bridge (ICB) connecting daughter cells to regulate abscission. Inhibition of Piezo1 caused multinucleation both in vitro and in vivo. Piezo1 positioning at the ICB during cytokinesis depends on Pacsin3. Pharmacological and genetic inhibition of Piezo1 or Pacsin3 resulted in mislocation of Rab11-family-interacting protein 3 (Rab11-FIP3) endosomes, apoptosis-linked gene 2-interacting protein X (ALIX), and endosomal sorting complex required for transport III (ESCRT-III). Furthermore, we identified FIP3 as the link between Piezo1-generated Ca2+ signals and ALIX delivery to the ICB, where ALIX recruits the ESCRT-III component charged multivesicular body protein 4B, which promotes abscission. These results provide a different view of how mechanical forces participate in cytokinesis and identify Piezo1 as a key modulator of endosome trafficking.
• dc.description.sponsorship This study was supported by the Spanish Ministry of Science, Education, and Universities through grants RTI2018-099718-B-100 to M.A.V. and PGC2018-095663-B100 to C.P., an institutional “María de Maeztu” Programme for Units of Excellence in R&D, and FEDER funds. C.P. is the recipient of an ICREA Academia award.
• dc.format.mimetype application/pdf
• dc.identifier.citation Carrillo-Garcia J, Herrera-Fernández V, Serra SA, Rubio-Moscardo F, Vogel-Gonzalez M, Doñate-Macian P, Hevia CF, Pujades C, Valverde MA. The mechanosensitive Piezo1 channel controls endosome trafficking for an efficient cytokinetic abscission. Sci Adv. 2021;7(44):eabi7785. DOI: 10.1126/sciadv.abi7785
• dc.identifier.doi http://dx.doi.org/10.1126/sciadv.abi7785
• dc.identifier.issn 2375-2548
• dc.identifier.uri http://hdl.handle.net/10230/49236
• dc.language.iso eng
• dc.publisher American Association for the Advancement of Science (AAAS)
• dc.relation.ispartof Sci Adv. 2021;7(44):eabi7785
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/RTI2018-099718-B-100
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PGC2018-095663-B100
• dc.rights © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S.Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri https://creativecommons.org/licenses/by-nc/4.0/
• dc.title The mechanosensitive Piezo1 channel controls endosome trafficking for an efficient cytokinetic abscission
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion