Analysis of alternative splicing during the combinatorial response to simultaneous copper and iron deficiency in arabidopsis reveals differential events in genes involved in amino acid metabolism

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• dc.contributor.author Mancini, Estefanía
• dc.contributor.author Garcia-Molina, Antoni
• dc.date.accessioned 2022-04-07T06:10:17Z
• dc.date.available 2022-04-07T06:10:17Z
• dc.date.issued 2022
• dc.description.abstract Copper (Cu) and iron (Fe) constitute fundamental nutrients for plant biology but are often limited due to low bioavailability. Unlike responses to single Cu or Fe deprivation, the consequences of simultaneous Cu and Fe deficiency have not yet been fully deciphered. Previously, it was demonstrated that Cu and Fe deficiency applied in combination imposes transcriptome, proteome, and metabolome changes different from those triggered under each deficiency individually. Here, we evaluated the effect of alternative splicing (AS) on the transcriptome of rosette leaves under single and simultaneous Cu and Fe deficiency. Differentially spliced genes (DSGs) and differentially expressed genes (DEGs) coincided in number (2,600 approx.) although the overlapping fraction was minimal (15%). Functional annotation of changes exclusively detected under simultaneous Cu and Fe deficiency revealed that DEGs participated in general stress responses and translation, while DSGs were involved in metabolic reactions, especially amino acid biosynthesis. Interestingly, transcripts encoding central features for tryptophan (Trp) and asparagine (Asn) synthesis - two significantly altered metabolites under simultaneous Cu and Fe deficiency - underwent exclusive intron retention events under the double deficiency. However, transcript and protein amounts for these enzymes did not correlate with Trp and Asn concentration. In consequence, we propose that AS might act as a regulatory mechanism to modify the stability and/or functionality of the enzymes and therefore fine-tune amino acid production during the combinatorial response to simultaneous Cu and Fe deficiency.
• dc.description.sponsorship This work was also supported by grant CEX2019-000902-S funded by MCIN/AEI/10.13039/501100011033 and by the CERCA Programme/Generalitat de Catalunya
• dc.format.mimetype application/pdf
• dc.identifier.citation Estefania Mancini, Antoni Garcia-Molina. Analysis of alternative splicing during the combinatorial response to simultaneous copper and iron deficiency in arabidopsis reveals differential events in genes involved in amino acid metabolism. Front Plant Sci. 2022 Jan 31;13:827828. DOI: 10.3389/fpls.2022.827828
• dc.identifier.doi http://dx.doi.org/10.3389/fimmu.2022.833548
• dc.identifier.issn 1664-462X
• dc.identifier.uri http://hdl.handle.net/10230/52838
• dc.language.iso eng
• dc.publisher Frontiers Media
• dc.relation.projectID https://creativecommons.org/licenses/by/4.0/
• dc.rights © 2022 Estefania Mancini and Antoni Garcia-Molina. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.subject.other Arabidopsis thaliana
• dc.subject.other Dèficit de coure
• dc.subject.other Dèficit de ferro
• dc.subject.other Genètica
• dc.title Analysis of alternative splicing during the combinatorial response to simultaneous copper and iron deficiency in arabidopsis reveals differential events in genes involved in amino acid metabolism
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion