Phenotypic variability in a coinfection with three independent Candida parapsilosis lineages

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  • dc.contributor.author Gómez-Molero, Emilia
  • dc.contributor.author Willis, Jesse R.
  • dc.contributor.author Dudakova, Anna
  • dc.contributor.author Carreté Muñoz, Laia, 1990-
  • dc.contributor.author Weig, Michael
  • dc.contributor.author Groß, Uwe
  • dc.contributor.author Gácser, Attila
  • dc.contributor.author Gabaldón Estevan, Juan Antonio, 1973-
  • dc.contributor.author Bader, Olivier
  • dc.date.accessioned 2020-11-10T07:05:47Z
  • dc.date.available 2020-11-10T07:05:47Z
  • dc.date.issued 2020
  • dc.description.abstract The human pathogenic yeast Candida parapsilosis has gained significant importance over the past decades as one of the principal causes of fungal bloodstream infections. Isolates of C. parapsilosis are known to be able to switch between several different colony morphologies in vitro, which are correlated with different cell shapes, altered cell surface properties, and thus different capacities to form biofilms on indwelling medical devices. In a set of six clinical specimens from a single surgery patient yielding stable smooth- as well as crepe-morphology isolates, we investigated the differences between five of them on a phenotypic and genomic level. In contrast to the initial assumption that they were switched forms of a clonal strain, karyotyping and genome sequencing showed that the patient was colonized by at least three distinct linages. Statistical analysis placed these groups distantly across the population of C. parapsilosis. Interestingly the single blood culture isolate was of smooth morphology and matched with an isolate from the patient's nose of similar morphology. Strong variation between the isolates was seen in adhesin-encoding genes, where repeat regions showed significant variation in length and repeat-numbers, most strikingly in HWP1 of the smooth isolates. Although no differences in drug susceptibility were evident, the high phylogenetic distance separating the individual strains highlights the need for testing of multiple colonies in routine practice. The absence of biofilm formation in the blood stream isolate indicates a lack of respective adhesins in the cell wall, in turn pointing toward lack of adhesion as a positively contributing factor for dissemination.
  • dc.description.sponsorship This study was funded through the FP7-PEOPLE-2013-ITN—Marie-Curie Action: “Initial Training Networks”: Molecular Mechanisms of Human Fungal Pathogen Host Interaction, ImResFun, Project ID: 606786, to AG, TG, OB, and UG.
  • dc.format.mimetype application/pdf
  • dc.identifier.citation Gómez-Molero E, Willis JR, Dudakova A, Carreté L, Weig M, Groß U, Gácser A, Gabaldón T, Bader O. Phenotypic variability in a coinfection with three independent Candida parapsilosis lineages. Front Microbiol. 2020; 11:1994. DOI: 10.3389/fmicb.2020.01994
  • dc.identifier.doi http://dx.doi.org/10.3389/fmicb.2020.01994
  • dc.identifier.issn 1664-302X
  • dc.identifier.uri http://hdl.handle.net/10230/45695
  • dc.language.iso eng
  • dc.publisher Frontiers
  • dc.relation.ispartof Front Microbiol. 2020; 11:1994
  • dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/606786
  • dc.rights © 2020 Gómez-Molero, Willis, Dudakova, Carreté, Weig, Groß, Gácser, Gabaldón and Bader. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.rights.uri http://creativecommons.org/licenses/by/4.0/
  • dc.subject.keyword Candida parapsilosis
  • dc.subject.keyword Adhesin genes
  • dc.subject.keyword Coinfection
  • dc.subject.keyword Genome sequencing
  • dc.subject.keyword Phenotypic variation
  • dc.title Phenotypic variability in a coinfection with three independent Candida parapsilosis lineages
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/publishedVersion

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