Defined chromosome structure in the genome-reduced bacterium Mycoplasma pneumoniae

Trussart, Marie, 1985-; Yus, Eva; Martínez, Sira; Baù, Davide; Tahara, Yuhei O.; Pengo, Thomas; Widjaja, Michael; Kretschmer, Simon; Swoger, Jim; Djordjevic, Steven P.; Turnbull, Lynne; Whitchurch, Cynthia B.; Miyata, Makoto; Martí Renom, Marc A.; Lluch-Senar, Maria 1982-; Serrano Pubull, Luis, 1982-

Defined chromosome structure in the genome-reduced bacterium Mycoplasma pneumoniae

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dc.contributor.author Trussart, Marie, 1985-ca
dc.contributor.author Yus, Evaca
dc.contributor.author Martínez, Siraca
dc.contributor.author Baù, Davideca
dc.contributor.author Tahara, Yuhei O.ca
dc.contributor.author Pengo, Thomasca
dc.contributor.author Widjaja, Michaelca
dc.contributor.author Kretschmer, Simonca
dc.contributor.author Swoger, Jimca
dc.contributor.author Djordjevic, Steven P.ca
dc.contributor.author Turnbull, Lynneca
dc.contributor.author Whitchurch, Cynthia B.ca
dc.contributor.author Miyata, Makotoca
dc.contributor.author Martí Renom, Marc A.ca
dc.contributor.author Lluch-Senar, Maria 1982-ca
dc.contributor.author Serrano Pubull, Luis, 1982-ca
dc.date.accessioned 2018-06-26T08:13:05Z
dc.date.available 2018-06-26T08:13:05Z
dc.date.issued 2017
dc.description.abstract DNA-binding proteins are central regulators of chromosome organization; however, in genome-reduced bacteria their diversity is largely diminished. Whether the chromosomes of such bacteria adopt defined three-dimensional structures remains unexplored. Here we combine Hi-C and super-resolution microscopy to determine the structure of the Mycoplasma pneumoniae chromosome at a 10 kb resolution. We find a defined structure, with a global symmetry between two arms that connect opposite poles, one bearing the chromosomal Ori and the other the midpoint. Analysis of local structures at a 3 kb resolution indicates that the chromosome is organized into domains ranging from 15 to 33 kb. We provide evidence that genes within the same domain tend to be co-regulated, suggesting that chromosome organization influences transcriptional regulation, and that supercoiling regulates local organization. This study extends the current understanding of bacterial genome organization and demonstrates that a defined chromosomal structure is a universal feature of living systems.
dc.description.sponsorship The research leading to these results was funded by the European Union Seventh Framework Programme (FP7/2007-2013 to L.S.), through the European Research Council, under grant agreement 232913 to L.S. and 609989 to M.A.M-R., the European Union's Horizon 2020 research and innovation program under Grant Agreement No. 634942 to L.S, the Fundación Botín to L.S., the Spanish Ministry of Economy and Competitiveness (BIO2007-61762 to L.S. and BFU2013-47736-P to M.A.M.-R., the National Plan of R+D+i, the ISCIII-Subdirección General de Evaluación y Fomento de la Investigación- (PI10/01702 to L.S.), the Human Frontiers Science Program (RGP0044 to M.A.M.-R.), the ERASynBio/MINECO Grant PCIN-2015-125 to L.S, and the European Regional Development Fund (ERDF) to L.S. We acknowledge support from the Spanish Ministry of Economy and Competitiveness, 'Centro de Excelencia Severo Ochoa 2013-2017' (SEV-2012-0208). We acknowledge the support of the CERCA Programme / Generalitat de Catalunya.
dc.format.mimetype application/pdf
dc.identifier.citation Trussart M, Yus E, Martinez S, Baù D, Tahara YO, Pengo T et al. Defined chromosome structure in the genome-reduced bacterium Mycoplasma pneumoniae. Nat Commun. 2017 Mar 8;8:14665. DOI: 10.1038/ncomms14665
dc.identifier.doi http://dx.doi.org/10.1038/ncomms14665
dc.identifier.issn 2041-1723
dc.identifier.uri http://hdl.handle.net/10230/34968
dc.language.iso eng
dc.publisher Nature Publishing Groupca
dc.relation.ispartof Nature Communications. 2017 Mar 8;8:14665
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/232913
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/609989
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/634942
dc.relation.projectID info:eu-repo/grantAgreement/ES/2PN/BIO2007-61762
dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2013–47736-P
dc.rights © The Author(s) 2017. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.subject.keyword Chromosome structures
dc.subject.keyword Mycoplasma pneumoniae
dc.subject.keyword Molecular modelling
dc.subject.keyword Alveolar rhabdomyosarcoma
dc.subject.keyword Pneumonia
dc.title Defined chromosome structure in the genome-reduced bacterium Mycoplasma pneumoniaeca
dc.type info:eu-repo/semantics/article
dc.type.version info:eu-repo/semantics/publishedVersion

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