Golgi-dependent transport of vacuolar sorting receptors is regulated by COPII, AP1, and AP4 protein complexes in tobacco

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• dc.contributor.author Gershlick, David Charles
• dc.contributor.author de Marcos Lousa, Carine
• dc.contributor.author Foresti, Ombretta
• dc.contributor.author Lee, Andrew J.
• dc.contributor.author Pereira, Estela A.
• dc.contributor.author Pinto-daSilva, Luis L.
• dc.contributor.author Bottanelli, Francesca
• dc.contributor.author Denecke, Jurgen
• dc.date.accessioned 2024-01-15T12:25:20Z
• dc.date.available 2024-01-15T12:25:20Z
• dc.date.issued 2014
• dc.description.abstract The cycling of vacuolar sorting receptors (VSRs) between early and late secretory pathway compartments is regulated by signals in the cytosolic tail, but the exact pathway is controversial. Here, we show that receptor targeting in tobacco (Nicotiana tabacum) initially involves a canonical coat protein complex II–dependent endoplasmic reticulum-to-Golgi bulk flow route and that VSR–ligand interactions in the cis-Golgi play an important role in vacuolar sorting. We also show that a conserved Glu is required but not sufficient for rate-limiting YXXɸ-mediated receptor trafficking. Protein–protein interaction studies show that the VSR tail interacts with the μ-subunits of plant or mammalian clathrin adaptor complex AP1 and plant AP4 but not that of plant and mammalian AP2. Mutants causing a detour of full-length receptors via the cell surface invariantly cause the secretion of VSR ligands. Therefore, we propose that cycling via the plasma membrane is unlikely to play a role in biosynthetic vacuolar sorting under normal physiological conditions and that the conserved Ile-Met motif is mainly used to recover mistargeted receptors. This occurs via a fundamentally different pathway from the prevacuolar compartment that does not mediate recycling. The role of clathrin and clathrin-independent pathways in vacuolar targeting is discussed.
• dc.format.mimetype application/pdf
• dc.identifier.citation Gershlick DC, Lousa CdM, Foresti O, Lee AJ, Pereira EA, daSilva LL, et al. Golgi-dependent transport of vacuolar sorting receptors is regulated by COPII, AP1, and AP4 protein complexes in tobacco. The Plant Cell. 2014 Mar;26(3):1308-29. DOI: 10.1105/tpc.113.122226
• dc.identifier.doi http://dx.doi.org/10.1105/tpc.113.122226
• dc.identifier.issn 1040-4651
• dc.identifier.uri http://hdl.handle.net/10230/58695
• dc.language.iso eng
• dc.publisher Oxford University Press
• dc.relation.ispartof The Plant Cell. 2014 Mar;26(3):1308-29
• dc.rights © 2014 American Society of Plant Biologists. All rights reserved. © The Author(s) 2014. Published by Oxford University Press on behalf of American Society of Plant Biologists. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.other Cèl·lules
• dc.subject.other Proteïnes
• dc.subject.other Plantes
• dc.title Golgi-dependent transport of vacuolar sorting receptors is regulated by COPII, AP1, and AP4 protein complexes in tobacco
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion