Stable multi-infection of splenocytes during SIV infection - the basis for/ncontinuous recombination
Mostra el registre complet Registre parcial de l'ítem
- dc.contributor.author Schultz, Ankeca
- dc.contributor.author Sopper, Sieghartca
- dc.contributor.author Sauermann, Ulrikeca
- dc.contributor.author Meyerhans, Andreasca
- dc.contributor.author Suspène, Rodolpheca
- dc.date.accessioned 2015-03-10T10:40:26Z
- dc.date.available 2015-03-10T10:40:26Z
- dc.date.issued 2012ca
- dc.description.abstract BACKGROUND: Recombination is an important mechanism in the generation of genetic diversity of the human (HIV) and simian (SIV) immunodeficiency viruses. It requires the co-packaging of divergent RNA genomes into the same retroviral capsid and subsequent template switching during the reverse transcription reaction. By HIV-specific fluorescence in situ hybridization (FISH), we have previously shown that the splenocytes from 2 chronically infected patients with Castelman's disease were multi-infected and thus fulfill the in vivo requirements to generate genetic diversity by recombination. In order to analyze when multi-infection first occurs during a lentivirus infection and how the distribution of multi-infection evolves during the disease course, we now determined the SIV copy numbers from splenocytes of 11 SIVmac251-infected rhesus macaques cross-sectionally covering the time span of primary infection throughout to end-stage immunodeficiency. RESULTS: SIV multi-infection of single splenocytes was readily detected in all monkeys and all stages of the infection. Single-infected cells were more frequent than double- or triple- infected cells. There was no strong trend linking the copy number distribution to plasma viral load, disease stage, or CD4 cell counts. CONCLUSIONS: SIV multi-infection of single cells is already established during the primary infection phase thus enabling recombination to affect viral evolution in vivo throughout the disease course.en
- dc.description.sponsorship AM was supported by grants from the Deutsche Forschungsgemeinschaft and the Spanish Ministry of Science and Innovation (SAF2010-21336)en
- dc.format.mimetype application/pdfca
- dc.identifier.citation Schultz A, Sopper S, Sauermann U, Meyerhans A, Suspène R. Stable multi-infection of splenocytes during SIV infection - the basis for/ncontinuous recombination. Retrovirology. 2012; 9: 31. DOI 10.1186/1742-4690-9-31ca
- dc.identifier.doi http://dx.doi.org/10.1186/1742-4690-9-31
- dc.identifier.issn 1742-4690ca
- dc.identifier.uri http://hdl.handle.net/10230/23175
- dc.language.iso engca
- dc.publisher BioMed Centralca
- dc.relation.ispartof Retrovirology. 2012; 9: 31
- dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/SAF2010-21336
- dc.rights © 2012 Schultz et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.ca
- dc.rights.accessRights info:eu-repo/semantics/openAccessca
- dc.rights.uri http://creativecommons.org/licenses/by/2.0
- dc.subject.keyword SIVen
- dc.subject.keyword FISHen
- dc.subject.keyword Recombinationen
- dc.subject.keyword Provirus copy numberen
- dc.subject.other Recombinació genèticaca
- dc.title Stable multi-infection of splenocytes during SIV infection - the basis for/ncontinuous recombinationca
- dc.type info:eu-repo/semantics/articleca
- dc.type.version info:eu-repo/semantics/publishedVersionca