Gene regulatory networks of epidermal and neural fate choice in a chordate

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• dc.contributor.author Leon, Anthony
• dc.contributor.author Subirana, Lucie
• dc.contributor.author Magre, Kevin
• dc.contributor.author Cases, Ildefonso
• dc.contributor.author Tena, Juan J.
• dc.contributor.author Irimia Martínez, Manuel
• dc.contributor.author Gómez Skarmeta, José Luis
• dc.contributor.author Escriva, Hector
• dc.contributor.author Bertrand, Stéphanie
• dc.date.accessioned 2022-05-30T10:28:41Z
• dc.date.available 2022-05-30T10:28:41Z
• dc.date.issued 2022
• dc.description.abstract Neurons are a highly specialized cell type only found in metazoans. They can be scattered throughout the body or grouped together, forming ganglia or nerve cords. During embryogenesis, centralized nervous systems develop from the ectoderm, which also forms the epidermis. How pluripotent ectodermal cells are directed toward neural or epidermal fates, and to which extent this process is shared among different animal lineages, are still open questions. Here, by using micromere explants, we were able to define in silico the putative gene regulatory networks (GRNs) underlying the first steps of the epidermis and the central nervous system formation in the cephalochordate amphioxus. We propose that although the signal triggering neural induction in amphioxus (i.e., Nodal) is different from vertebrates, the main transcription factors implicated in this process are conserved. Moreover, our data reveal that transcription factors of the neural program seem to not only activate neural genes but also to potentially have direct inputs into the epidermal GRN, suggesting that the Nodal signal might also contribute to neural fate commitment by repressing the epidermal program. Our functional data on whole embryos support this result and highlight the complex interactions among the transcription factors activated by the signaling pathways that drive ectodermal cell fate choice in chordates.
• dc.description.sponsorship The work was supported by the Centre Nationale de la Recherche Scientifique, by the “Agence Nationale de la Recherche” (grants ANR-19-CE13-0011-01 and ANR-16-CE12-0008-01 to H.E.) and by the European project Assemble Plus (H2020-INFRAIA-1-2016-2017; grant no. 730984 to H.E.). Also was supported by the European Research Council (grant ERC-StG-LS2-637591 to M.I.) and by the Spanish Ministerio de Ciencia (grant BFU2017-89201-P to M.I. and grant PID2019-103921GB-I00 to J.J.T.)
• dc.format.mimetype application/pdf
• dc.identifier.citation Leon A, Subirana L, Magre K, Cases I, Tena JJ, Irimia M et al. Gene regulatory networks of epidermal and neural fate choice in a chordate. Mol Biol Evol. 2022 Apr 11;39(4):msac055. DOI:10.1093/molbev/msac055
• dc.identifier.doi http://dx.doi.org/10.1093/molbev/msac055
• dc.identifier.issn 0737-4038
• dc.identifier.uri http://hdl.handle.net/10230/53314
• dc.language.iso eng
• dc.publisher Oxford University Press
• dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/730984
• dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/637591
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/BFU2017-89201-P
• dc.rights © Anthony Leon et al. 2022. Published by Oxford University Press on behalf of Society for Molecular Biology and Evolution. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by-nc-/4.0/
• dc.subject.other Genètica
• dc.subject.other Epidermis
• dc.subject.other Sistema nerviós central
• dc.title Gene regulatory networks of epidermal and neural fate choice in a chordate
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion