An amphioxus neurula stage cell atlas supports a complex scenario for the emergence of vertebrate head mesoderm

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• dc.contributor.author Grau Bové, Xavier
• dc.contributor.author Subirana, Lucie
• dc.contributor.author Meister, Lydvina
• dc.contributor.author Soubigou, Anaël
• dc.contributor.author Neto, Ana
• dc.contributor.author Elek, Anamaria
• dc.contributor.author Naranjo, Silvia
• dc.contributor.author Fornas Carreño, Oscar
• dc.contributor.author Gómez Skarmeta, José Luis
• dc.contributor.author Tena, Juan J.
• dc.contributor.author Irimia Martínez, Manuel
• dc.contributor.author Bertrand, Stéphanie
• dc.contributor.author Sebé-Pedrós, Arnau
• dc.contributor.author Escriva, Hector
• dc.date.accessioned 2024-07-16T06:38:17Z
• dc.date.available 2024-07-16T06:38:17Z
• dc.date.issued 2024
• dc.description.abstract The emergence of new structures can often be linked to the evolution of novel cell types that follows the rewiring of developmental gene regulatory subnetworks. Vertebrates are characterized by a complex body plan compared to the other chordate clades and the question remains of whether and how the emergence of vertebrate morphological innovations can be related to the appearance of new embryonic cell populations. We previously proposed, by studying mesoderm development in the cephalochordate amphioxus, a scenario for the evolution of the vertebrate head mesoderm. To further test this scenario at the cell population level, we used scRNA-seq to construct a cell atlas of the amphioxus neurula, stage at which the main mesodermal compartments are specified. Our data allowed us to validate the presence of a prechordal-plate like territory in amphioxus. Additionally, the transcriptomic profile of somite cell populations supports the homology between specific territories of amphioxus somites and vertebrate cranial/pharyngeal and lateral plate mesoderm. Finally, our work provides evidence that the appearance of the specific mesodermal structures of the vertebrate head was associated to both segregation of pre-existing cell populations, and co-option of new genes for the control of myogenesis.
• dc.description.sponsorship We would like to thank Jose Luis Ferran for advices on the revision. This work benefited from access to the Observatoire Océanologique de Banyuls-sur-Mer, an EMBRC-France and EMBRC-ERIC site. Embryo imaging experiments were undertaken using the material of the BIOPIC platform. The laboratory of H.E. and S.B. was supported by the CNRS, and by the “Agence Nationale de la Recheche” under the grants ANR-19-CE13-0011 to H.E. and ANR-21-CE13-0034 to S.B. Research in A.S-P. group was supported by the European Research Council (ERC-StG 851647 to A.S-P.) and the Spanish Ministry of Science and Innovation (PID2021-124757NB-I00 to A.S-P.). X.G-B. is supported by the European Union’s H2020 research and innovation program under Marie Sklodowska-Curie grant agreement 101031767 to X.G-B. A.E. was supported by FPI PhD fellowships from the Spanish Ministry of Science and Innovation. J.J.T. was supported by the Spanish Ministerio de Economía y Competitividad (grant PID2019-103921GB-I00 and PID2022-141288NB-I00 to J.T.). M.I. laboratory research has been funded by the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (ERCCoG-LS2-101002275 to M.I.), by the Spanish Ministry of Economy and Competitiveness (PID2020-115040GB-I00 to M.I.) and by the ‘Centro de Excelencia Severo Ochoa 2013-2017’(SEV-2012-0208 to M. I.).
• dc.format.mimetype application/pdf
• dc.identifier.citation Grau-Bové X, Subirana L, Meister L, Soubigou A, Neto A, Elek A, et al. An amphioxus neurula stage cell atlas supports a complex scenario for the emergence of vertebrate head mesoderm. Nat Commun. 2024 May 29;15(1):4550. DOI: 10.1038/s41467-024-48774-4
• dc.identifier.doi http://dx.doi.org/10.1038/s41467-024-48774-4
• dc.identifier.issn 2041-1723
• dc.identifier.uri http://hdl.handle.net/10230/60759
• dc.language.iso eng
• dc.publisher Nature Research
• dc.relation.ispartof Nat Commun. 2024 May 29;15(1):4550
• dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/851647
• dc.relation.projectID info:eu-repo/grantAgreement/ES/3PE/PID2021-124757NB-I00
• dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/101031767
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PID2019-103921GB-I00
• dc.relation.projectID info:eu-repo/grantAgreement/ES/3PE/PID2022-141288NB-I00
• dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/101002275
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PID2020-115040GB-I00
• dc.rights © The Author(s) 2024. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Cell lineage
• dc.subject.keyword Evolutionary developmental biology
• dc.subject.keyword Neurulation
• dc.title An amphioxus neurula stage cell atlas supports a complex scenario for the emergence of vertebrate head mesoderm
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion