Prenatal exposure to mixtures of xenoestrogens and repetitive element DNA methylation changes in human placenta

Vilahur Chiaraviglio, Nadia, 1982-; Bustamante Pineda, Mariona; Byun, Hyang-Min; Fernandez, Mariana F.; Santa Marina, Loreto; Basterrechea, Mikel; Ballester Díez, Ferran; Murcia, Mario; Tardón, Adonina; Fernández Somoano, Ana; Estivill, Xavier, 1955-; Olea, Nicolás; Sunyer Deu, Jordi; Baccarelli, Andrea A.

Prenatal exposure to mixtures of xenoestrogens and repetitive element DNA methylation changes in human placenta

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dc.contributor.author Vilahur Chiaraviglio, Nadia, 1982-ca
dc.contributor.author Bustamante Pineda, Marionaca
dc.contributor.author Byun, Hyang-Minca
dc.contributor.author Fernandez, Mariana F.ca
dc.contributor.author Santa Marina, Loretoca
dc.contributor.author Basterrechea, Mikelca
dc.contributor.author Ballester Díez, Ferranca
dc.contributor.author Murcia, Marioca
dc.contributor.author Tardón, Adoninaca
dc.contributor.author Fernández Somoano, Anaca
dc.contributor.author Estivill, Xavier, 1955-ca
dc.contributor.author Olea, Nicolásca
dc.contributor.author Sunyer Deu, Jordica
dc.contributor.author Baccarelli, Andrea A.ca
dc.date.accessioned 2016-06-13T08:02:05Z
dc.date.available 2016-10-01T02:00:03Z
dc.date.issued 2014ca
dc.description.abstract BACKGROUND: Prenatal exposure to endocrine disrupting compounds (EDCs) has previously shown to alter epigenetic marks. OBJECTIVES: In this work we explore whether prenatal exposure to mixtures of xenoestrogens has the potential to alter the placenta epigenome, by studying DNA methylation in retrotransposons as a surrogate of global DNA methylation. METHODS: The biomarker total effective xenoestrogen burden (TEXB) was measured in 192 placentas from participants in the longitudinal INMA Project. DNA methylation was quantitatively assessed by bisulfite pyrosequencing on 10 different retrotransposons including 3 different long interspersed nuclear elements (LINEs), 4 short interspersed nuclear elements (SINEs) and 3 human endogenous retroviruses (HERVs). Associations were tested using linear mixed-effects regression models and sex interaction was evaluated.RESULTS: A significant sex interaction was observed for AluYb8 (p-value for interaction <0.001, significant at Bonferroni corrected p-value threshold of 0.0025). Boys with the highest TEXB-alpha levels of exposure (third tertile) presented on average a decrease of 0.84% in methylation compared to those in the first tertile (p-value<0.001), while no significant effects were found in girls (p-value=0.134). CONCLUSIONS: Our findings suggest that boys may be more susceptible to the effect of exposure to xenoestrogens during prenatal development, producing shifts in DNA methylation of certain sensitive genomic repetitive sequences in a tissue important for fetal growth and development.
dc.description.sponsorship This work was supported by grants from the Spanish Ministry of Health (FIS-PI042018; FIS-PI060867; FIS-PI081151; FIS-PI09/02311; FIS-PI09/02647; FIS-PI11/00610); Instituto de Salud Carlos III [Red INMA G03/176 and CB06/02/0041]; the EU Commission (QLK4-1999-01422, QLK4-2002-00603 and CONTAMED FP7-ENV-212502), the Generalitat de Catalunya—CIRIT [1999SGR 00241]; the Consejería de Salud de la Junta de Andalucía (183/07 and 0675/10), the Diputación Foral de Gipuzkoa (DFG06/004), the Department of Health of the Basque Government (2005111093), and by the University of Oviedo, Obra Social Cajastur, the Fundación Roger Torné and La Fundació La Marató de TV3. NV was supported by an FPI Grant from the Spanish Ministry of Health (BES-2009-023933) and a Formación de Personal Investigador Grant for Short Research Stays in Foreign Institutions (BES-2009-023933). AA Baccarelli receives support from the Harvard School of Public Health and National Institute of Environmental Health Sciences Center for Environmental Health (R01 ES021357).The HUSC BioBank, integrated in the Andalusia Public Health System (SSPA) and the National Biobank Network, is financed by the Institute of Health Carlos III, (RD09/0076/00148) and the Regional Government of Andalucia
dc.format.mimetype application/pdfca
dc.identifier.citation Vilahur N, Bustamante M, Byun HM, Fernandez MF, Santa Marina L, Basterrechea M et al. Prenatal exposure to mixtures of xenoestrogens and repetitive element DNA methylation changes in human placenta. Environ Int. 2014 Oct;71:81-7. DOI: 10.1016/j.envint.2014.06.006ca
dc.identifier.doi http://dx.doi.org/10.1016/j.envint.2014.06.006
dc.identifier.issn 0160-4120ca
dc.identifier.uri http://hdl.handle.net/10230/26908
dc.language.iso engca
dc.publisher Elsevierca
dc.relation.ispartof Environment International. 2014 Oct;71:81-7
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/212502
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.subject.other Embaràs -- Complicacions
dc.subject.other Metilació
dc.subject.other Placenta -- Metabolisme
dc.title Prenatal exposure to mixtures of xenoestrogens and repetitive element DNA methylation changes in human placentaca
dc.type info:eu-repo/semantics/articleca
dc.type.version info:eu-repo/semantics/acceptedVersionca

Col·leccions

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