bsAS, an antisense long non-coding RNA, essential for correct wing development through regulation of blistered/DSRF isoform usage

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  • dc.contributor.author Pérez-Lluch, Sílvia
  • dc.contributor.author Klein, Cecilia C.
  • dc.contributor.author Breschi, Alessandra, 1988-
  • dc.contributor.author Ruiz-Romero, Marina
  • dc.contributor.author Abad, Amaya
  • dc.contributor.author Palumbo, Emilio
  • dc.contributor.author Bekish, Lyazzat
  • dc.contributor.author Arnan Ros, Carme
  • dc.contributor.author Guigó Serra, Roderic
  • dc.date.accessioned 2021-02-17T06:51:29Z
  • dc.date.available 2021-02-17T06:51:29Z
  • dc.date.issued 2020
  • dc.description.abstract Natural Antisense Transcripts (NATs) are long non-coding RNAs (lncRNAs) that overlap coding genes in the opposite strand. NATs roles have been related to gene regulation through different mechanisms, including post-transcriptional RNA processing. With the aim to identify NATs with potential regulatory function during fly development, we generated RNA-Seq data in Drosophila developing tissues and found bsAS, one of the most highly expressed lncRNAs in the fly wing. bsAS is antisense to bs/DSRF, a gene involved in wing development and neural processes. bsAS plays a crucial role in the tissue specific regulation of the expression of the bs/DSRF isoforms. This regulation is essential for the correct determination of cell fate during Drosophila development, as bsAS knockouts show highly aberrant phenotypes. Regulation of bs isoform usage by bsAS is mediated by specific physical interactions between the promoters of these two genes, which suggests a regulatory mechanism involving the collision of RNA polymerases transcribing in opposite directions. Evolutionary analysis suggests that bsAS NAT emerged simultaneously to the long-short isoform structure of bs, preceding the emergence of wings in insects.
  • dc.description.sponsorship This work was supported by the European Community under the FP7 program (ERC-2011-AdG-294653-RNA-MAPS to R.G.), by the Spanish Ministry of Economy and Competitiveness (MEC) (BIO2011-26205 to R.G), by the Centro de Excelencia Severo Ochoa, from the CERCA Programme (Generalitat de Catalunya), and from the Spanish Ministry of Science and Innovation to the EMBL partnership. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
  • dc.format.mimetype application/pdf
  • dc.identifier.citation Pérez-Lluch S, Klein CC, Breschi A, Ruiz-Romero M, Abad A, Palumbo E, Bekish L, Arnan C, Guigó R. bsAS, an antisense long non-coding RNA, essential for correct wing development through regulation of blistered/DSRF isoform usage. PLoS Genet. 2020; 16(12):e1009245. DOI: 10.1371/journal.pgen.1009245
  • dc.identifier.doi http://dx.doi.org/10.1371/journal.pgen.1009245
  • dc.identifier.issn 1553-7390
  • dc.identifier.uri http://hdl.handle.net/10230/46505
  • dc.language.iso eng
  • dc.publisher Public Library of Science (PLoS)
  • dc.relation.ispartof PLoS Genet. 2020; 16(12):e1009245
  • dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/294653
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BIO2011-26205
  • dc.rights © 2020 Pérez-Lluch et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.rights.uri http://creativecommons.org/licenses/by/4.0/
  • dc.subject.keyword Long non-coding RNA
  • dc.subject.keyword Eyes
  • dc.subject.keyword Imaginal discs
  • dc.subject.keyword Drosophila melanogaster
  • dc.subject.keyword Invertebrate genomics
  • dc.subject.keyword Larvae
  • dc.subject.keyword Animal wings
  • dc.subject.keyword Gene expression
  • dc.title bsAS, an antisense long non-coding RNA, essential for correct wing development through regulation of blistered/DSRF isoform usage
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/publishedVersion

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