Neuroanatomical characterization of the Nmu-Cre knock-in mice reveals an interconnected network of unique neuropeptidergic cells

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• dc.contributor.author Medrano, Mireia
• dc.contributor.author Allaoui, Wissal
• dc.contributor.author Van Bulck, Mathias
• dc.contributor.author Thys, Sofie
• dc.contributor.author Makrini-Maleville, Leila
• dc.contributor.author Seuntjens, Eve
• dc.contributor.author De Vos, Winnok H.
• dc.contributor.author Valjent, Emmanuel
• dc.contributor.author Gaszner, Bálazs
• dc.contributor.author Van Eeckhaut, Ann
• dc.contributor.author Smolders, Ilse
• dc.contributor.author De Bundel, Dimitri
• dc.date.accessioned 2025-05-05T06:14:13Z
• dc.date.available 2025-05-05T06:14:13Z
• dc.date.issued 2023
• dc.description.abstract Neuromedin U (NMU) is an evolutionary conserved neuropeptide that has been implicated in multiple processes, such as circadian regulation, energy homeostasis, reward processing and stress coping. Although the central expression of NMU has been addressed previously, the lack of specific and sensitive tools has prevented a comprehensive characterization of NMU-expressing neurons in the brain. We have generated a knock-in mouse model constitutively expressing Cre recombinase under the Nmu promoter. We have validated the model using a multi-level approach based on quantitative reverse-transcription polymerase chain reactions, in situ hybridization, a reporter mouse line and an adenoviral vector driving Cre-dependent expression of a fluorescent protein. Using the Nmu-Cre mouse, we performed a complete characterization of NMU expression in adult mouse brain, unveiling a potential midline NMU modulatory circuit with the ventromedial hypothalamic nucleus (VMH) as a key node. Moreover, immunohistochemical analysis suggested that NMU neurons in the VMH mainly constitute a unique population of hypothalamic cells. Taken together, our results suggest that Cre expression in the Nmu-Cre mouse model largely reflects NMU expression in the adult mouse brain, without altering endogenous NMU expression. Thus, the Nmu-Cre mouse model is a powerful and sensitive tool to explore the role of NMU neurons in mice.en
• dc.format.mimetype application/pdf
• dc.identifier.citation Medrano M, Allaoui W, Van Bulck M, Thys S, Makrini-Maleville L, Seuntjens E, et al. Neuroanatomical characterization of the Nmu-Cre knock-in mice reveals an interconnected network of unique neuropeptidergic cells. Open Biol. 2023 Jun;13(6):220353. DOI: 10.1098/rsob.220353
• dc.identifier.doi http://dx.doi.org/10.1098/rsob.220353
• dc.identifier.issn 2046-2441
• dc.identifier.uri http://hdl.handle.net/10230/70267
• dc.language.iso eng
• dc.publisher Royal Society
• dc.relation.ispartof Open Biology. 2023 Jun;13(6):220353
• dc.rights © 2023 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Neuromedin Uen
• dc.subject.keyword Neuropeptideen
• dc.subject.keyword Knock-in mouse modelen
• dc.subject.keyword Ventromedial hypothalamic nucleusen
• dc.title Neuroanatomical characterization of the Nmu-Cre knock-in mice reveals an interconnected network of unique neuropeptidergic cellsen
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion