A physical mechanism of TANGO1-mediated bulky cargo export

Mostra el registre complet Registre parcial de l'ítem

  • dc.contributor.author Raote, Ishier
  • dc.contributor.author Chabanon, Morgan
  • dc.contributor.author Walani, Nikhil
  • dc.contributor.author Arroyo, Marino
  • dc.contributor.author Garcia-Parajo, Maria F.
  • dc.contributor.author Malhotra, Vivek
  • dc.contributor.author Campelo, Felix
  • dc.date.accessioned 2020-12-16T07:12:08Z
  • dc.date.available 2020-12-16T07:12:08Z
  • dc.date.issued 2020
  • dc.description.abstract The endoplasmic reticulum (ER)-resident protein TANGO1 assembles into a ring around ER exit sites (ERES), and links procollagens in the ER lumen to COPII machinery, tethers, and ER-Golgi intermediate compartment (ERGIC) in the cytoplasm (Raote et al., 2018). Here, we present a theoretical approach to investigate the physical mechanisms of TANGO1 ring assembly and how COPII polymerization, membrane tension, and force facilitate the formation of a transport intermediate for procollagen export. Our results indicate that a TANGO1 ring, by acting as a linactant, stabilizes the open neck of a nascent COPII bud. Elongation of such a bud into a transport intermediate commensurate with bulky procollagens is then facilitated by two complementary mechanisms: (i) by relieving membrane tension, possibly by TANGO1-mediated fusion of retrograde ERGIC membranes and (ii) by force application. Altogether, our theoretical approach identifies key biophysical events in TANGO1-driven procollagen export.
  • dc.description.sponsorship M Chabanon, MF Garcia-Parajo and F Campelo acknowledge support from the Government of Spain (FIS2015-63550-R, FIS2017-89560-R, BFU2015-73288-JIN, RYC-2017–22227, and PID2019-106232RB-I00/10.13039/501100011033; Severo Ochoa CEX2019-000910-S), Fundació Cellex, Fundació Mir-Puig, and Generalitat de Catalunya (CERCA, AGAUR), ERC Advanced Grant NANO-MEMEC (GA 788546) and LaserLab 4 Europe (GA 654148). I Raote and V Malhotra acknowledge funding by grants from the Ministerio de Economía, Industria y Competitividad Plan Nacional (BFU2013-44188-P) and Consolider (CSD2009-00016); support of the Spanish Ministry of Economy and Competitiveness, through the Programmes ‘Centro de Excelencia Severo Ochoa 2013–2017’ (SEV-2012–0208) and Maria de Maeztu Units of Excellence in R and D (MDM-2015–0502); and support of the CERCA Programme/Generalitat de Catalunya. I Raote, MF Garcia-Parajo, V Malhotra., and F Campelo acknowledge initial support by a BIST Ignite Grant (eTANGO). I Raote acknowledges support from the Spanish Ministry of Science, Innovation and Universities (IJCI-2017–34751). M Arroyo and N Walani acknowledge the support of the European Research Council (CoG-681434), and M Arroyo that of the Generalitat de Catalunya (2017-SGR-1278 and ICREA Academia prize for excellence in research) and of the Spanish Ministry of Economy and Competitiveness, through the Severo Ochoa Programme (CEX2018-000797- S)
  • dc.format.mimetype application/pdf
  • dc.identifier.citation Raote I, Chabanon M,Walani N, Arroyo M, Garcia-Parajo MF, Malhotra M et al. A physical mechanism of TANGO1-mediated bulky cargo export. Elife. 2020 Nov 10;9:e59426. DOI: 10.7554/eLife.59426
  • dc.identifier.doi http://dx.doi.org/10.7554/eLife.59426
  • dc.identifier.issn 2050-084X
  • dc.identifier.uri http://hdl.handle.net/10230/46059
  • dc.language.iso eng
  • dc.publisher eLife
  • dc.relation.ispartof Elife. 2020 Nov 10;9:e59426
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2013-44188-P
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2015-73288-JIN
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/IJCI-2017-34751
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/RYC-2017-22227
  • dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/788546
  • dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/654148
  • dc.rights © 2020, Raote et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.rights.uri https://creativecommons.org/licenses/by/4.0/
  • dc.subject.other Citologia
  • dc.subject.other Proteïnes
  • dc.title A physical mechanism of TANGO1-mediated bulky cargo export
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/publishedVersion

Col·leccions

Articles (Center for Genomic Regulation (CRG))
Articles (Departament de Medicina i Ciències de la Vida)
Documents OpenAIRE (Open Access Infrastructure for Research in Europe)