A descriptive analysis of urinary ESBL-producing- Escherichia coli in Cerdanya Hospital
Mostra el registre complet Registre parcial de l'ítem
- dc.contributor.author Gaviria, Loreno Patrícia
- dc.contributor.author Montsant, Lourdes
- dc.contributor.author Azuaje, Carlos
- dc.contributor.author González-Díaz, Aida
- dc.contributor.author Horcajada Gallego, Juan Pablo
- dc.contributor.author Limón, Enric
- dc.contributor.author Viñas, Miguel
- dc.contributor.author Espinal, Paula
- dc.contributor.author Fusté, Ester
- dc.date.accessioned 2022-10-10T06:16:53Z
- dc.date.available 2022-10-10T06:16:53Z
- dc.date.issued 2022
- dc.description.abstract Urinary tract infections caused by extended-spectrum β-lactamase Escherichia coli (ESBL-EC) are increasing worldwide and are a current concern because treatment options are often limited. This study investigated antimicrobial susceptibility, antimicrobial resistance genes (ARGs), and the biological diversity of urinary ESBL-EC isolates at Cerdanya Hospital, a European cross-border hospital that combines French and Spanish healthcare models. Bacterial identification and susceptibility were determined using the Microscan WalkAway® system and ESBL production was examined by the double-disk synergy method. Isolates were sequenced using the Ion S5™ next-generation sequencing system, with the whole-genome sequences then assembled using SPADEs software and analyzed using PubMLST, ResFinder, FimTyper, PlasmidFinder, and VirulenceFinder. A phylogenetic analysis was performed by constructing an assembly-based core-SNV alignment, followed by a phylogenetic tree constructed using Parsnp from the Harvest suite. All isolates studied were multidrug-resistant and could be classified into 19 different sequence types characterized by a high genetic diversity. The most prevalent ESBL-enzymes were CTX-M-14 and CTX-M-15. High-risk international clones (ST131, ST10, and ST405) were also identified. The results demonstrated the absence of a single predominant clone of ESBL-MDR-EC at Cerdanya Hospital.
- dc.format.mimetype application/pdf
- dc.identifier.citation Gaviria LP, Montsant L, Azuaje C, González-Díaz A, Horcajada JP, Limón E, Viñas M, Espinal P, Fusté E. A descriptive analysis of urinary ESBL-producing- Escherichia coli in Cerdanya Hospital. Microorganisms. 2022 Feb 22;10(3):488. DOI: 10.3390/microorganisms10030488
- dc.identifier.doi http://dx.doi.org/10.3390/microorganisms10030488
- dc.identifier.issn 2076-2607
- dc.identifier.uri http://hdl.handle.net/10230/54319
- dc.language.iso eng
- dc.publisher MDPI
- dc.relation.ispartof Microorganisms. 2022 Feb 22;10(3):488
- dc.rights © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri https://creativecommons.org/licenses/by/4.0/
- dc.subject.keyword Escherichia coli
- dc.subject.keyword Extended-spectrum β-lactamase (ESBL)
- dc.subject.keyword Molecular characterization
- dc.subject.keyword Multidrug resistance (MDR)
- dc.subject.keyword Sequence type (ST)
- dc.subject.keyword Urinary tract infections (UTI)
- dc.subject.keyword Whole-genome sequencing (WGS)
- dc.title A descriptive analysis of urinary ESBL-producing- Escherichia coli in Cerdanya Hospital
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion