Upon Wnt stimulation, Rac1 activation requires Rac1 and Vav2 binding to p120-catenin
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- dc.contributor.author Valls, Gabrielaca
- dc.contributor.author Codina, Montserratca
- dc.contributor.author Miller, Rachel K.ca
- dc.contributor.author Valle Pérez, Beatriz delca
- dc.contributor.author Vinyoles, Meritxellca
- dc.contributor.author Caelles Franch, Carmeca
- dc.contributor.author McCrea, Pierre D.ca
- dc.contributor.author García de Herreros, Antonioca
- dc.contributor.author Duñach, Mireiaca
- dc.date.accessioned 2015-12-10T18:49:29Z
- dc.date.available 2015-12-10T18:49:29Z
- dc.date.issued 2012
- dc.description.abstract A role for Rac1 GTPase in canonical Wnt signaling has recently been demonstrated, showing that it is required for β-catenin translocation to the nucleus. In this study, we investigated the mechanism of Rac1 stimulation by Wnt. Upregulation of Rac1 activity by Wnt3a temporally correlated with enhanced p120-catenin binding to Rac1 and Vav2. Vav2 and Rac1 association with p120-catenin was modulated by phosphorylation of this protein, which was stimulated upon serine/threonine phosphorylation by CK1 and inhibited by tyrosine phosphorylation by Src or Fyn. Acting on these two post-translational modifications, Wnt3a induced the release of p120-catenin from E-cadherin, enabled the interaction of p120-catenin with Vav2 and Rac1, and facilitated Rac1 activation by Vav2. Given that p120-catenin depletion disrupts gastrulation in Xenopus, we analyzed p120-catenin mutants for their ability to rescue this phenotype. In contrast to the wild-type protein or other controls, p120-catenin point mutants that were deficient in the release from E-cadherin or in Vav2 or Rac1 binding failed to rescue p120-catenin depletion. Collectively, these results indicate that binding of p120-catenin to Vav2 and Rac1 is required for the activation of this GTPase upon Wnt signaling.ca
- dc.format.mimetype application/pdfca
- dc.identifier.citation Valls G, Codina M, Miller RK, Del Valle-Pérez B, Vinyoles M, Caelles C et al. Upon Wnt stimulation, Rac1 activation requires Rac1 and Vav2 binding to p120-catenin. Journal of cell science. 2012;125(Pt 22):5288-301. DOI: 10.1242/jcs.101030ca
- dc.identifier.doi http://dx.doi.org/10.1242/jcs.101030
- dc.identifier.issn 0021-9525
- dc.identifier.uri http://hdl.handle.net/10230/25374
- dc.language.iso engca
- dc.publisher Company of Biologistsca
- dc.relation.ispartof Journal of cell science. 2012;125(Pt 22):5288-301
- dc.rights © Company of Biologistsca
- dc.rights.accessRights info:eu-repo/semantics/openAccessca
- dc.subject.other Fosforilacióca
- dc.subject.other Xenopus laevis -- Embriologiaca
- dc.title Upon Wnt stimulation, Rac1 activation requires Rac1 and Vav2 binding to p120-cateninca
- dc.type info:eu-repo/semantics/articleca
- dc.type.version info:eu-repo/semantics/publishedVersionca