Real time and spatiotemporal quantification of pH and H2O2 imbalances with a multiplex surface-enhanced raman spectroscopy nanosensor

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• dc.contributor.author Xiao, Can, 1988-
• dc.contributor.author Izquierdo-Roca, Victor
• dc.contributor.author Rivera Gil, Pilar, 1976-
• dc.date.accessioned 2023-03-10T06:54:40Z
• dc.date.available 2023-03-10T06:54:40Z
• dc.date.issued 2023
• dc.description.abstract Oxidative stress is involved in many aging-related pathological disorders and is the result of defective cellular management of redox reactions. Particularly, hydrogen peroxide (H2O2), is a major byproduct and a common oxidative stress biomarker. Monitoring its dynamics and a direct correlation to diseases remains a challenge due to the complexity of redox reactions. Sensitivity and specificity are major drawbacks for H2O2 sensors regardless of their readout. Luminiscent boronate-based probes such as 3-mercaptophenylboronic acid (3-MPBA) are emerging as the most effective quantitation tool due to their specificity and sensitivity. Problems associated with these probes are limited intracellular sensing, water solubility, selectivity, and quenching. We have synthesized a boronate-based nanosensor with a surface-enhanced Raman spectroscopy (SERS) readout to solve these challenges. Furthermore, we found out that environmental pH gradients, as found in biological samples, affect the sensitivity of boronate-based sensors. When the sensor is in an alkaline environment, the oxidation of 3-MPBA by H2O2 is more favored than in an acidic environment. This leads to different H2O2 measurements depending on pH. To solve this issue, we synthesized a multiplex nanosensor capable of concomitantly quantifying pH and H2O2. Our nanosensor first measures the local pH and based on this value, provides the amount of H2O2. It seems that this pH-dependent sensitivity effect applies to all boronic acid based probes. We tested the multiplexing ability by quantitatively measuring intra- and extracellular pH and H2O2 dynamics under physiological and pathological conditions on healthy cells and cells in which H+ and/or H2O2 homeostasis has been altered.
• dc.description.sponsorship P.R.G. acknowledges the Ministry of Science, Innovation and Universities (MICINN-AEI) (AEI-PID2019-106755RB-I00, RYC-2012-10059, CEX2018-000792-M) and the AGAUR (2017 SGR 1054 and 2021PROD00041) for financial support. C.X. and P.R.G. appreciate the financial support from China Scholarship Council (CSC) (201609110104).
• dc.format.mimetype application/pdf
• dc.identifier.citation Xiao C, Izquierdo-Roca V, Rivera-Gil P. Real time and spatiotemporal quantification of pH and H2O2 imbalances with a multiplex surface-enhanced raman spectroscopy nanosensor. ACS Mater Au. 2023;3(2):164–75. DOI: 10.1021/acsmaterialsau.2c00069
• dc.identifier.doi http://dx.doi.org/10.1021/acsmaterialsau.2c00069
• dc.identifier.issn 2694-2461
• dc.identifier.uri http://hdl.handle.net/10230/56137
• dc.language.iso eng
• dc.publisher American Chemical Society (ACS)
• dc.relation.ispartof ACS Mater Au. 2023;3(2):164–75
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PID2019-106755RB-I00
• dc.rights © 2023 The Authors 2021. Published by American Chemical Society. This work is licensed under a Creative Commons Attribution 4.0 International License.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri https://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Colloidal plasmonic nanocapsules
• dc.subject.keyword Surface-enhanced Raman scattering
• dc.subject.keyword Aromatic boronic acid sensors
• dc.subject.keyword pH and H2O2 biosensing
• dc.subject.keyword Multiplex biosensors
• dc.subject.keyword Cell homeostasis
• dc.title Real time and spatiotemporal quantification of pH and H2O2 imbalances with a multiplex surface-enhanced raman spectroscopy nanosensor
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion