Hindbrain rhombomere centers harbor a heterogenous population of dividing progenitors which rely on Notch signaling

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• dc.contributor.author Belmonte Mateos, Carla, 1992-
• dc.contributor.author Meister, Lydvina
• dc.contributor.author Pujades Corbi, Cristina
• dc.date.accessioned 2023-12-13T08:37:56Z
• dc.date.available 2023-12-13T08:37:56Z
• dc.date.issued 2023
• dc.description.abstract Tissue growth and morphogenesis are interrelated processes, whose tight coordination is essential for the production of different cell fates and the timely precise allocation of stem cell capacities. The zebrafish embryonic brainstem, the hindbrain, exemplifies such coupling between spatiotemporal cell diversity acquisition and tissue growth as the neurogenic commitment is differentially distributed over time. Here, we combined cell lineage and in vivo imaging approaches to reveal the emergence of specific cell population properties within the rhombomeres. We studied the molecular identity of hindbrain rhombomere centers and showed that they harbor different progenitor capacities that change over time. By clonal analysis, we revealed that cells within the center of rhombomeres decrease the proliferative capacity to remain mainly in the G1 phase. Proliferating progenitors give rise to neurons by asymmetric and symmetric neurogenic divisions while maintaining the pool of progenitors. The proliferative capacity of these cells differs from their neighbors, and they are delayed in the onset of Notch activity. Through functional studies, we demonstrated that they rely on Notch3 signaling to be maintained as non-committed progenitors. In this study, we show that cells in rhombomere centers, despite the neurogenic asynchrony, might share steps of a similar program with the rhombomere counterparts, to ensure proper tissue growth.
• dc.description.sponsorship The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This work was funded by grants BFU2015-67400-P, PGC2018-095663-B-I00, and PID2021-123261NB-I00 from Ministerio de Ciencia e Innovación (MICIN), Agencia Estatal de Investigación (AEI, DOI: 10.13039/501100011033), and Fondo Europeo de Desarrollo Regional (FEDER) to CP. The Department of Medicine and Life Sciences (UPF) is a Unidad de Excelencia María de Maeztu (CEX2018-000792-M) funded by MICIN and AEI. CBM was the recipient of a predoctoral fellowship from MICIN (FPI, BES-2016-076664). CP is a recipient of the ICREA Academia award (Generalitat de Catalunya).
• dc.format.mimetype application/pdf
• dc.identifier.citation Belmonte-Mateos C, Meister L, Pujades C. Hindbrain rhombomere centers harbor a heterogenous population of dividing progenitors which rely on Notch signaling. Front Cell Dev Biol. 2023 Nov 2;11:1268631. DOI: 10.3389/fcell.2023.1268631
• dc.identifier.doi http://dx.doi.org/10.3389/fcell.2023.1268631
• dc.identifier.issn 2296-634X
• dc.identifier.uri http://hdl.handle.net/10230/58523
• dc.language.iso eng
• dc.publisher Frontiers
• dc.relation.ispartof Front Cell Dev Biol. 2023 Nov 2;11:1268631
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2015-67400-P
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PGC2018-095663-B-I00
• dc.relation.projectID info:eu-repo/grantAgreement/ES/3PE/PID2021-123261NB-I00
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/CEX2018-000792-M
• dc.rights © 2023 Belmonte-Mateos, Meister and Pujades. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Notch signaling
• dc.subject.keyword Cell division mode
• dc.subject.keyword Hindbrain rhombomeres
• dc.subject.keyword Neural progenitors
• dc.subject.keyword Neurogenesis
• dc.subject.keyword Zebrafish
• dc.title Hindbrain rhombomere centers harbor a heterogenous population of dividing progenitors which rely on Notch signaling
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion