Delta9-tetrahydrocannabinol modulates the proteasome system in the brain

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• dc.contributor.author Salgado Mendialdúa, Victòriaca
• dc.contributor.author Aguirre Plans, Joaquim, 1993-ca
• dc.contributor.author Guney, Emreca
• dc.contributor.author Reig-Viader, Ritaca
• dc.contributor.author Maldonado, Rafael, 1961-ca
• dc.contributor.author Bayés, Angelsca
• dc.contributor.author Oliva Miguel, Baldomeroca
• dc.contributor.author Ozaita Mintegui, Andrés, 1969-ca
• dc.date.accessioned 2018-09-17T08:10:17Z
• dc.date.issued 2018
• dc.description.abstract Cannabis is the most consumed illicit drug worldwide. Its principal psychoactive component, Δ9-tetrahydrocannabinol (THC), affects multiple brain functions, including cognitive performance, by modulating cannabinoid type-1 (CB1) receptors. These receptors are strongly enriched in presynaptic terminals, where they modulate neurotransmitter release. We analyzed, through a proteomic screening of hippocampal synaptosomal fractions, those proteins and pathways modulated 3 h after a single administration of an amnesic dose of THC (10 mg/kg, i.p.). Using an isobaric labeling approach, we identified 2040 proteins, 1911 of them previously reported in synaptic proteomes, confirming the synaptic content enrichment of the samples. Initial analysis revealed a significant alteration of 122 proteins, where 42 increased and 80 decreased their expression. Gene set enrichment analysis indicated an over-representation of mitochondrial associated functions and cellular metabolic processes. A second analysis focusing on extreme changes revealed 28 proteins with altered expression after THC treatment, 15 of them up-regulated and 13 down-regulated. Using a network topology-based scoring algorithm we identified those proteins in the mouse proteome with the greatest association to the 28 modulated proteins. This analysis pinpointed a significant alteration of the proteasome function, since top scoring proteins were related to the proteasome system (PS), a protein complex involved in ATP-dependent protein degradation. In this regard, we observed that THC decreases 20S proteasome chymotrypsin-like protease activity in the hippocampus. Our data describe for the first time the modulation of the PS in the hippocampus following THC administration under amnesic conditions that may contribute to an aberrant plasticity at synapses.
• dc.description.sponsorship This study was supported by Grants from the Ministerio de Economía, Innovación y Competitividad (MINECO) (#BFU2015-68568-P (MINECO/FEDER, UE) to A.O., #BFU2015-69717-P (MINECO/FEDER, UE) to RRV and AB, #RYC-2011-08391 to AB, #SAF2017-90664-REDT to RRV and AB, #SAF2014-59648-P and #SAF2017-84060-R to R.M.; a grant from the Instituto de Salud Carlos III (#RD16/0017/0020) to R.M.; Generalitat de Catalunya (2017SGR-669 to R.M. and 2017SGR1776 to RRV and AB) and ICREA (Institució Catalana de Recerca i Estudis Avançats) Academia to A.O. and R.M. Mass spectrometric analyses were performed at the CRG/UPF Proteomics Unit which is part of the of Proteored, PRB3 and is supported by grant PT17/0019, of the PE I + D + i 2013-2016, funded by ISCIII and ERDF. Grant “Unidad de Excelencia María de Maeztu”, funded by the MINECO (#MDM-2014-0370); PLAN E (Plan Español para el Estímulo de la Economía y el Empleo); FEDER funding is also acknowledged
• dc.format.mimetype application/pdf
• dc.identifier.citation Salgado-Mendialdúa V, Aguirre-Plans J, Guney E, Reig-Viader R, Maldonado R, Bayés À et al. Delta9-tetrahydrocannabinol modulates the proteasome system in the brain. Biochem Pharmacol. 2018;157:159-68. DOI: 10.1016/j.bcp.2018.08.026
• dc.identifier.doi http://dx.doi.org/10.1016/j.bcp.2018.08.026
• dc.identifier.issn 0006-2952
• dc.identifier.uri http://hdl.handle.net/10230/35452
• dc.language.iso eng
• dc.publisher Elsevierca
• dc.relation.ispartof Biochemical Pharmacology. 2018;157:159-68
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2015-68568-P
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2015-69717-P
• dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/RYC-2011-08391
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/SAF2017-90664-REDT
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/SAF2014-59648-P
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/SAF2017-84060-R
• dc.rights © Elsevier http://dx.doi.org/10.1016/j.bcp.2018.08.026
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.subject.other Cannabinoides
• dc.subject.other Hipocamp
• dc.subject.other Proteasoma
• dc.title Delta9-tetrahydrocannabinol modulates the proteasome system in the brainca
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/acceptedVersion