Physiological control of nitric oxide in neuronal BACE1 translation by heme-regulated eIF2α kinase HRI induces synaptogenesis

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• dc.contributor.author Ill-Raga, Gerard, 1982-ca
• dc.contributor.author Tajes Orduña, Martaca
• dc.contributor.author Busquets Garcia, Arnau, 1985-ca
• dc.contributor.author Ramos Fernández, Eva, 1984-ca
• dc.contributor.author Vargas, Lina M.ca
• dc.contributor.author Bosch Morató, Mònica, 1986-ca
• dc.contributor.author Guivernau Almazán, Biuse, 1988-ca
• dc.contributor.author Valls Comamala, Victòria, 1987-ca
• dc.contributor.author Eraso Pichot, Abelca
• dc.contributor.author Guix Ràfols, Francesc Xavierca
• dc.contributor.author Fandos, Césarca
• dc.contributor.author Rosen, Mark D.ca
• dc.contributor.author Rabinowitz, Michael H.ca
• dc.contributor.author Maldonado, Rafael, 1961-ca
• dc.contributor.author Álvarez, Alejandra R.ca
• dc.contributor.author Ozaita Mintegui, Andrés, 1969-ca
• dc.contributor.author Muñoz López, Francisco José, 1964-ca
• dc.date.accessioned 2016-06-10T14:26:09Z
• dc.date.available 2016-06-10T14:26:09Z
• dc.date.issued 2015
• dc.description.abstract AIMS: Hippocampus is the brain center for memory formation, a process that requires synaptogenesis. However, hippocampus is dramatically compromised in Alzheimer's disease due to the accumulation of amyloid β-peptide, whose production is initiated by β-site APP Cleaving Enzyme 1 (BACE1). It is known that pathological stressors activate BACE1 translation through the phosphorylation of the eukaryotic initiation factor-2α (eIF2α) by GCN2, PERK, or PKR kinases, leading to amyloidogenesis. However, BACE1 physiological regulation is still unclear. Since nitric oxide (NO) participates directly in hippocampal glutamatergic signaling, we investigated the neuronal role of the heme-regulated eukaryotic initiation factor eIF2α kinase (HRI), which can bind NO by a heme group, in BACE1 translation and its physiological consequences. RESULTS: We found that BACE1 is expressed on glutamate activation with NO being the downstream effector by triggering eIF2α phosphorylation, as it was obtained by Western blot and luciferase assay. It is due to the activation of HRI by NO as assayed by Western blot and immunofluorescence with an HRI inhibitor and HRI siRNA. BACE1 expression was early detected at synaptic spines, contributing to spine growth and consolidating the hippocampal memory as assayed with mice treated with HRI or neuronal NO synthase inhibitors. INNOVATION: We provide the first description that HRI and eIF2α are working in physiological conditions in the brain under the control of nitric oxide and glutamate signaling, and also that BACE1 has a physiological role in hippocampal function. CONCLUSION: We conclude that BACE1 translation is controlled by NO through HRI in glutamatergic hippocampal synapses, where it plays physiological functions, allowing the spine growth and memory consolidation.ca
• dc.description.sponsorship Supported by the Plan Estatal de I+D+i 2013-2016 and the ISCIII-Subdirección General de Evaluación y Fomento de la Investigación (Grants PI13/00408, BFU2012-33500 and Red HERACLES RD12/0042/0014) and FEDER Funds; Generalitat de Catalunya (SGR09-1369); and Fundació la Marató de TV3 (100310).
• dc.format.mimetype application/pdfca
• dc.identifier.citation Ill-Raga G, Tajes M, Busquets-García A, Ramos-Fernández E, Vargas LM, Bosch-Morató M et al. Physiological control of nitric oxide in neuronal BACE1 translation by heme-regulated eIF2α kinase HRI induces synaptogenesis. Antioxidants & redox signaling. 2015; 22(15): 1295-1307. DOI 10.1089/ars.2014.6080ca
• dc.identifier.doi http://dx.doi.org/10.1089/ars.2014.6080
• dc.identifier.issn 1523-0864
• dc.identifier.uri http://hdl.handle.net/10230/26904
• dc.language.iso engca
• dc.publisher Mary Ann Liebert, Incca
• dc.relation.ispartof Antioxidants & redox signaling. 2015; 22(15): 1295-1307
• dc.rights Final publication is available from Mary Ann Liebert, Inc., publishers http://dx.doi.org/10.1089/ars.2014.6080ca
• dc.rights.accessRights info:eu-repo/semantics/openAccessca
• dc.subject.keyword Alzheimer’s disease
• dc.subject.keyword BACE1
• dc.subject.keyword eIF2-alpha
• dc.subject.keyword Nitric oxide
• dc.subject.keyword HRI
• dc.subject.keyword Protein translation
• dc.subject.other Òxid nítricca
• dc.subject.other Alzheimer, Malaltia d'ca
• dc.title Physiological control of nitric oxide in neuronal BACE1 translation by heme-regulated eIF2α kinase HRI induces synaptogenesisca
• dc.type info:eu-repo/semantics/articleca
• dc.type.version info:eu-repo/semantics/acceptedVersionca