MCRS1 modulates the heterogeneity of microtubule minus-end morphologies in mitotic spindles

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  • dc.contributor.author Laguillo Diego, Alejandra, 1991-
  • dc.contributor.author Kiewisz, Robert
  • dc.contributor.author Martí-Gómez, Carlos
  • dc.contributor.author Baum, Daniel
  • dc.contributor.author Müller-Reichert, Thomas
  • dc.contributor.author Vernos, Isabelle, 1959-
  • dc.date.accessioned 2023-02-24T07:00:43Z
  • dc.date.available 2023-02-24T07:00:43Z
  • dc.date.issued 2023
  • dc.description.abstract Faithful chromosome segregation requires the assembly of a bipolar spindle, consisting of two antiparallel microtubule (MT) arrays having most of their minus ends focused at the spindle poles and their plus ends overlapping in the spindle midzone. Spindle assembly, chromosome alignment, and segregation require highly dynamic MTs. The plus ends of MTs have been extensively investigated but their minus-end structure remains poorly characterized. Here, we used large-scale electron tomography to study the morphology of the MT minus ends in three dimensionally reconstructed metaphase spindles in HeLa cells. In contrast to the homogeneous open morphology of the MT plus ends at the kinetochores, we found that MT minus ends are heterogeneous, showing either open or closed morphologies. Silencing the minus end-specific stabilizer, MCRS1 increased the proportion of open MT minus ends. Altogether, these data suggest a correlation between the morphology and the dynamic state of the MT ends. Taking this heterogeneity of the MT minus-end morphologies into account, our work indicates an unsynchronized behavior of MTs at the spindle poles, thus laying the groundwork for further studies on the complexity of MT dynamics regulation.
  • dc.description.sponsorship We acknowledge support from the European Union’s Horizon 2020 research and innovation programme under Marie Skłodowska-Curie grant agreement No. 675737 (DiviDE ITN network) to A.L.D., R.K., I.V., and T.M.-R. Research in the Müller-Reichert laboratory is supported by funds from the Deutsche Forschungsgemeinschaft (MU 1423/8-2). Work in the Vernos lab was supported by Spanish Ministry of Economy (MINECO) I+D grant BFU2012-37163 and BFU2015-68726-P. A.L.D also received an EMBO short-term fellowship to visit the Müller-Reichert lab, grant agreement No. 8704. We thank Tobias Fürstenhaupt (Electron Microscopy Facility at the MPI-CBG, Dresden, Germany) for technical support. We also thank the Vernos and Müller-Reichert groups and the members of the DiviDE ITN for discussions. We acknowledge the support of the Spanish Ministry of Economy, Industry and Competitiveness (MEIC) to the CRG-EMBL partnership and support of the Spanish Ministry of Economy and Competitiveness, “Centro de Excelencia Severo Ochoa,” as well as support of the CERCA Programme/Generalitat de Catalunya.”
  • dc.format.mimetype application/pdf
  • dc.identifier.citation Laguillo-Diego A, Kiewisz R, Martí-Gómez C, Baum D, Müller-Reichert T, Vernos I. MCRS1 modulates the heterogeneity of microtubule minus-end morphologies in mitotic spindles. Mol Biol Cell. 2023 Jan 1;34(1):ar1. DOI: 10.1091/mbc.E22-08-0306-T
  • dc.identifier.doi http://dx.doi.org/10.1091/mbc.E22-08-0306-T
  • dc.identifier.issn 1059-1524
  • dc.identifier.uri http://hdl.handle.net/10230/55902
  • dc.language.iso eng
  • dc.publisher American Society for Cell Biology
  • dc.relation.ispartof Mol Biol Cell. 2023 Jan 1;34(1):ar1
  • dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/675737
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2012-37163
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2015-68726-P
  • dc.rights © 2023 Laguillo-Diego, Kiewisz, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). It is available to the public under an Attribution 4.0 International Creative Commons CC-BY 4.0 License (https://creativecommons.org/licenses/by/4.0/).
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.rights.uri https://creativecommons.org/licenses/by/4.0/
  • dc.title MCRS1 modulates the heterogeneity of microtubule minus-end morphologies in mitotic spindles
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/publishedVersion

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