Q-nexus: a comprehensive and efficient analysis pipeline designed for ChIP-nexus

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• dc.contributor.author Hansen, Peterca
• dc.contributor.author Hecht, Jochenca
• dc.contributor.author Ibn-Salem, Jonasca
• dc.contributor.author Menkuec, Benjamin S.ca
• dc.contributor.author Roskosch, Sebastianca
• dc.contributor.author Truss, Matthiasca
• dc.contributor.author Robinson, Peter N.ca
• dc.date.accessioned 2017-01-12T11:18:49Z
• dc.date.available 2017-01-12T11:18:49Z
• dc.date.issued 2016ca
• dc.description.abstract Background: ChIP-nexus, an extension of the ChIP-exo protocol, can be used to map the borders of protein-bound DNA sequences at nucleotide resolution, requires less input DNA and enables selective PCR duplicate removal using random barcodes. However, the use of random barcodes requires additional preprocessing of the mapping data, which complicates the computational analysis. To date, only a very limited number of software packages are available for the analysis of ChIP-exo data, which have not yet been systematically tested and compared on ChIP-nexus data. Results: Here, we present a comprehensive software package for ChIP-nexus data that exploits the random barcodes for selective removal of PCR duplicates and for quality control. Furthermore, we developed bespoke methods to estimate the width of the protected region resulting from protein-DNA binding and to infer binding positions from ChIP-nexus data. Finally, we applied our peak calling method as well as the two other methods MACE and MACS2 to the available ChIP-nexus data. Conclusions: The Q-nexus software is efficient and easy to use. Novel statistics about duplication rates in consideration of random barcodes are calculated. Our method for the estimation of the width of the protected region yields unbiased signatures that are highly reproducible for biological replicates and at the same time very specific for the respective factors analyzed. As judged by the irreproducible discovery rate (IDR), our peak calling algorithm shows a substantially better reproducibility. An implementation of Q-nexus is available at http://charite.github.io/Q/.
• dc.description.sponsorship This project was supported by the Bundesministerium für Bildung und Forschung (BMBF; project no. 0313911 and 13GW0099) and the European Community’s Seventh Framework Programme (grant agreement no. 602300; SYBIL). Furthermore, we acknowledge support of the Spanish Ministry of Economy and Competitiveness, ‘Centro de Excelencia Severo Ochoa 2013-2017’.
• dc.format.mimetype application/pdfca
• dc.identifier.citation Hansen P, Hecht J, Ibn-Salem J, Menkuec BS, Roskosch S, Truss M, Robinson PN. Q-nexus: a comprehensive and efficient analysis pipeline designed for ChIP-nexus. BMC Genomics. 2016;17:873. DOI: 10.1186/s12864-016-3164-6ca
• dc.identifier.doi http://dx.doi.org/10.1186/s12864-016-3164-6
• dc.identifier.issn 1471-2164ca
• dc.identifier.uri http://hdl.handle.net/10230/27873
• dc.language.iso engca
• dc.publisher BioMed Centralca
• dc.relation.ispartof BMC Genomics. 2016;17:873
• dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/602300
• dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/SEV2012-0208
• dc.rights © The Author(s). 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to theCreative Commons license, and indicate if changes were made.ca
• dc.rights.accessRights info:eu-repo/semantics/openAccessca
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Chromatin immunoprecipitation
• dc.subject.keyword ChIP-nexus
• dc.subject.keyword ChIP-exo
• dc.subject.keyword Duplication rates
• dc.subject.keyword Library complexity
• dc.subject.keyword Algorithm
• dc.subject.keyword Bioinformatics
• dc.title Q-nexus: a comprehensive and efficient analysis pipeline designed for ChIP-nexusca
• dc.type info:eu-repo/semantics/articleca
• dc.type.version info:eu-repo/semantics/publishedVersionca