Evolution of the early spliceosomal complex-from constitutive to regulated splicing

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• dc.contributor.author Borao Arriazu, Sonia, 1993-
• dc.contributor.author Ayté del Olmo, José
• dc.contributor.author Hümmer, Stefan
• dc.date.accessioned 2021-12-15T07:30:17Z
• dc.date.available 2021-12-15T07:30:17Z
• dc.date.issued 2021
• dc.description.abstract Pre-mRNA splicing is a major process in the regulated expression of genes in eukaryotes, and alternative splicing is used to generate different proteins from the same coding gene. Splicing is a catalytic process that removes introns and ligates exons to create the RNA sequence that codifies the final protein. While this is achieved in an autocatalytic process in ancestral group II introns in prokaryotes, the spliceosome has evolved during eukaryogenesis to assist in this process and to finally provide the opportunity for intron-specific splicing. In the early stage of splicing, the RNA 5' and 3' splice sites must be brought within proximity to correctly assemble the active spliceosome and perform the excision and ligation reactions. The assembly of this first complex, termed E-complex, is currently the least understood process. We focused in this review on the formation of the E-complex and compared its composition and function in three different organisms. We highlight the common ancestral mechanisms in S. cerevisiae, S. pombe, and mammals and conclude with a unifying model for intron definition in constitutive and regulated co-transcriptional splicing.
• dc.description.sponsorship This work and APC was funded by the Spanish Ministerio de Economia y Competitividad (MINECO), PLAN E and Feder and by Unidad de Excelencia María de Maeztu, grant numbers: BFU2018-PGC2018-097248-B-I00 and CEX2018-000792-M.
• dc.format.mimetype application/pdf
• dc.identifier.citation Borao S, Ayté J, Hümmer S. Evolution of the early spliceosomal complex-from constitutive to regulated splicing. Int J Mol Sci. 2021;22(22):12444. DOI: 10.3390/ijms222212444
• dc.identifier.doi http://dx.doi.org/10.3390/ijms222212444
• dc.identifier.issn 1422-0067
• dc.identifier.uri http://hdl.handle.net/10230/49210
• dc.language.iso eng
• dc.publisher MDPI
• dc.relation.ispartof Int J Mol Sci. 2021;22(22):12444
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PGC2018-097248-B-I00
• dc.rights © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword 5′ splicing site
• dc.subject.keyword E-complex
• dc.subject.keyword Prp2
• dc.subject.keyword U2AF65
• dc.subject.keyword Exon–intron junction
• dc.subject.keyword Fission yeast
• dc.subject.keyword Spliceosome
• dc.subject.keyword Splicing
• dc.title Evolution of the early spliceosomal complex-from constitutive to regulated splicing
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion