Generation of feeder-free pig induced pluripotent stem cells without Pou5f1

Montserrat Pulido, Núria; Oñate Monje, Lorena de, 1985-; Garreta Bahima, Elena; González, Federico; Adamo, Antonio; Eguizábal, Cristina; Häfner, Sophia; Vassena, Rita; Izpisúa Belmonte, J. C.

Generation of feeder-free pig induced pluripotent stem cells without Pou5f1

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dc.contributor.author Montserrat Pulido, Núriaca
dc.contributor.author Oñate Monje, Lorena de, 1985-ca
dc.contributor.author Garreta Bahima, Elenaca
dc.contributor.author González, Federicoca
dc.contributor.author Adamo, Antonioca
dc.contributor.author Eguizábal, Cristinaca
dc.contributor.author Häfner, Sophiaca
dc.contributor.author Vassena, Ritaca
dc.contributor.author Izpisúa Belmonte, J. C.ca
dc.date.accessioned 2017-01-04T08:28:15Z
dc.date.available 2017-01-04T08:28:15Z
dc.date.issued 2012ca
dc.description.abstract The pig represents an ideal large-animal model, intermediate between rodents and humans, for the preclinical assessment of emerging cell therapies. As no validated pig embryonic stem (pES) cell lines have been derived so far, pig induced pluripotent stem cells (piPSCs) should offer an alternative source of undifferentiated cells to advance regenerative medicine research from bench to clinical trial. We report here for the first time the derivation of piPSCs from adult fibroblast with only three transcription factors: Sox2 (sex determining region Y-box 2), Klf4 (Krüppel-like factor 4), and c-Myc (avian myelocytomatosis viral oncogene homolog). We have been able to demonstrate that exogenous Pou5f1 (POU domain class 5 transcription factor 1; abbreviated as Octamer-4: Oct4) is dispensable to achieve and maintain pluripotency in the generation of piPSCs. To the best of our knowledge, this is also the first report of somatic reprogramming in any species without the overexpression, either directly or indirectly, of Oct4. Moreover, we were able to generate piPSCs without the use of feeder cells, approaching thus xeno-free conditions. Our work paves the way for the derivation of clinical grade piPSCs for regenerative medicine.
dc.description.sponsorship NM was partially supported by Juan de la Cierva Program, EG was partially supported by Sara Borrell Program. This work was partially supported by grants from MINECO, Fondo de Investigaciones Sanitarias (TERCEL, PI052847), Fundación Cellex and the G. Harold and Leila Y. Mathers Charitable Foundation
dc.format.mimetype application/pdfca
dc.identifier.citation Montserrat N, de Oñate L, Garreta E, González F, Adamo A, Eguizábal C, Häfner S, Vassena R, Izpisua Belmonte JC. Generation of feeder-free pig induced pluripotent stem cells without Pou5f1. Cell Transplant. 2012; 21(5): 815-25. DOI: 10.3727/096368911X601019ca
dc.identifier.doi http://dx.doi.org/10.3727/096368911X601019
dc.identifier.issn 0963-6897ca
dc.identifier.uri http://hdl.handle.net/10230/27850
dc.language.iso engca
dc.publisher Cognizant Communication Corporationca
dc.relation.ispartof Cell Transplantation. 2012;21(5):815-25
dc.rights Article and/or figure(s) cannot be used for resale. Please use proper citation format when citing this article including the DOI, publisher reference, volume number and page locationca
dc.rights.accessRights info:eu-repo/semantics/openAccessca
dc.rights.uri https://creativecommons.org/licenses/by-nc/3.0/
dc.subject.other Cèl·lules mare
dc.subject.other Cél·lules mare embrionàries
dc.title Generation of feeder-free pig induced pluripotent stem cells without Pou5f1ca
dc.type info:eu-repo/semantics/articleca
dc.type.version info:eu-repo/semantics/publishedVersionca

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