Evaluation of triple negative breast cancer with heterogeneous immune infiltration
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- dc.contributor.author Quintana, Ángela
- dc.contributor.author Arenas, Enrique J.
- dc.contributor.author Bernadó Morales, Cristina
- dc.contributor.author Fernández Navarro, José
- dc.contributor.author González, Jonatan
- dc.contributor.author Esteve-Codina, Anna
- dc.contributor.author Moliné, Teresa
- dc.contributor.author Martí, Mercè
- dc.contributor.author Curigliano, Giuseppe
- dc.contributor.author Schmid, Peter
- dc.contributor.author Peg, Vicente
- dc.contributor.author Arribas, Joaquín
- dc.contributor.author Cortés, Javier
- dc.date.accessioned 2023-06-21T06:34:40Z
- dc.date.available 2023-06-21T06:34:40Z
- dc.date.issued 2023
- dc.description.abstract Introduction: Tumor infiltrating lymphocytes (TILs) are known to be a prognostic and predictive biomarker in breast cancer, particularly in triple negative breast cancer (TNBC) patients. International guidelines have been proposed to evaluate them in the clinical setting as a continuous variable, without a clear defined cut-off. However, there are scenarios where the immune infiltration is heterogeneous that some areas of the patient's tumour have high numbers of TILs while other areas completely lack them. This spontaneous presentation of a heterogeneous immune infiltration could be a great opportunity to study why some tumours present TILs at diagnosis but others do not, while eliminating inter patient's differences. Methods: In this study, we have identified five TNBC patients that showed great TIL heterogeneity, with areas of low (≤5%) and high (≥50%) numbers of TILs in their surgical specimens. To evaluate immune infiltration heterogeneity, we performed and analyzed bulk RNA-sequencing in three independent triplicates from the high and low TIL areas of each patient. Results: Gene expression was homogeneous within the triplicates in each area but was remarkable different between TILs regions. These differences were not only due to the presence of TILs as there were other non-inflammatory genes and pathways differentially expressed between the two areas. Discussion: This highlights the importance of intratumour heterogeneity driving the immune infiltration, and not patient's characteristics like the HLA phenotype, germline DNA or immune repertoire.
- dc.format.mimetype application/pdf
- dc.identifier.citation Quintana Á, Arenas EJ, Bernadó C, Fernández Navarro J, González J, Esteve-Codina A, Moliné T, Marti M, Curigliano G, Schmid P, Peg V, Arribas J, Cortés J. Evaluation of triple negative breast cancer with heterogeneous immune infiltration. Front Immunol. 2023 May 4;14:1149747. DOI: 10.3389/fimmu.2023.1149747
- dc.identifier.doi http://dx.doi.org/10.3389/fimmu.2023.1149747
- dc.identifier.issn 1664-3224
- dc.identifier.uri http://hdl.handle.net/10230/57282
- dc.language.iso eng
- dc.publisher Frontiers
- dc.relation.ispartof Front Immunol. 2023 May 4;14:1149747
- dc.rights © 2023 Quintana, Arenas, Bernadó, Navarro, González, Esteve-Codina, Moliné, Marti, Curigliano, Schmid, Peg, Arribas and Cortés. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri http://creativecommons.org/licenses/by/4.0/
- dc.subject.keyword Immune cell abundance
- dc.subject.keyword Intratumor heterogeneity
- dc.subject.keyword Transcriptomics
- dc.subject.keyword Triple negative breast cancer
- dc.subject.keyword Tumor-infiltrating lymphocytes
- dc.title Evaluation of triple negative breast cancer with heterogeneous immune infiltration
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion