Yap/Taz-TEAD activity links mechanical cues to progenitor cell behavior during zebrafish hindbrain segmentation

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• dc.contributor.author Voltes Cobo, Adrià, 1991-
• dc.contributor.author Hevia, Covadonga F.
• dc.contributor.author Engel-Pizcueta, Carolyn
• dc.contributor.author Dingare, Chaitanya
• dc.contributor.author Calzolari, Simone, 1983-
• dc.contributor.author Terriente, Javier
• dc.contributor.author Norden, Caren
• dc.contributor.author Lecaudey, Virginie
• dc.contributor.author Pujades Corbi, Cristina
• dc.date.accessioned 2019-09-16T14:20:46Z
• dc.date.issued 2019
• dc.description.abstract Cells perceive their microenvironment through chemical and physical cues. However, how the mechanical signals are interpreted during embryonic tissue deformation to result in specific cell behaviors is largely unknown. The Yap/Taz family of transcriptional co-activators has emerged as an important regulator of tissue growth and regeneration, responding to physical cues from the extracellular matrix, and to cell shape and actomyosin cytoskeletal changes. In this study, we demonstrate the role of Yap/Taz-TEAD activity as a sensor of mechanical signals in the regulation of the progenitor behavior of boundary cells during zebrafish hindbrain compartmentalization. Monitoring of in vivo Yap/Taz activity during hindbrain segmentation indicated that boundary cells responded to mechanical cues in a cell-autonomous manner through Yap/Taz-TEAD activity. Cell-lineage analysis revealed that Yap/Taz-TEAD boundary cells decreased their proliferative activity when Yap/Taz-TEAD activity ceased, which preceded changes in their cell fate from proliferating progenitors to differentiated neurons. Functional experiments demonstrated the pivotal role of Yap/Taz-TEAD signaling in maintaining progenitor features in the hindbrain boundary cell population.
• dc.description.sponsorship This work was supported by a La Marató-TV3 grant (345/C/2014) and by a Ministerio de Ciencia, Innovación y Universidades grant (BFU2015-67400-P and BFU2016-81887-REDT/AEI) to C.P.; and by a Unidad de Excelencia María de Maetzu grant (MDM-2014-0370) to the Department of Experimental and Health Sciences of the Pompeu Fabra University (DCEXS-UPF). A.V. was a recipient of a predoctoral fellowship from the Fundació La Caixa, and C.E.-P. holds a predoctoral fellowship from the Ministerio de Ciencia, Innovación y Universidades (FPU). J.T. was a recipient of a postdoctoral Beatriu de Pinos fellowship (AGAUR, Generalitat de Catalunya). C.P. is recipient of an Institució Catalana per la Recerca i Estudis Avançats Academia award (Generalitat de Catalunya).
• dc.format.mimetype application/pdf
• dc.identifier.citation Voltes A, Hevia CF, Engel-Pizcueta C, Dingare C, Calzolari S, Terriente J et al. Yap/Taz-TEAD activity links mechanical cues to progenitor cell behavior during zebrafish hindbrain segmentation. Development. 2019;146(14):dev176735. DOI: 10.1242/dev.176735
• dc.identifier.doi http://dx.doi.org/10.1242/dev.176735
• dc.identifier.issn 0950-1991
• dc.identifier.uri http://hdl.handle.net/10230/42278
• dc.language.iso eng
• dc.publisher Company of Biologists
• dc.relation.ispartof Development. 2019;146(14):dev176735
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2015-67400-P
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2016-81887-REDT
• dc.rights © Company of Biologists https://dev.biologists.org/content/146/14/dev176735 DOI 10.1242/dev.176735
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.subject.keyword Boundaries
• dc.subject.keyword Compartments
• dc.subject.keyword Hindbrain
• dc.subject.keyword Mechanical cues
• dc.subject.keyword Neurons
• dc.subject.keyword Progenitor cells
• dc.subject.keyword Yap/Taz
• dc.title Yap/Taz-TEAD activity links mechanical cues to progenitor cell behavior during zebrafish hindbrain segmentation
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion