Comparative analysis of vertebrates reveals that mouse primordial oocytes do not contain a Balbiani body
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- dc.contributor.author Dhandapani, Laasya
- dc.contributor.author Salzer, Marion C.
- dc.contributor.author Duran, Juan M.
- dc.contributor.author Zaffagnini, Gabriele
- dc.contributor.author De Guirior, Cristian
- dc.contributor.author Martínez-Zamora, Maria Angeles
- dc.contributor.author Böke, Elvan
- dc.date.accessioned 2022-01-13T06:43:14Z
- dc.date.issued 2022
- dc.description.abstract Oocytes spend the majority of their lifetime in a primordial state. The cellular and molecular biology of primordial oocytes is largely unexplored; yet, it is necessary to study them to understand the mechanisms through which oocytes maintain cellular fitness for decades, and why they eventually fail with age. Here, we develop enabling methods for live-imaging-based comparative characterization of Xenopus, mouse and human primordial oocytes. We show that primordial oocytes in all three vertebrate species contain active mitochondria, Golgi and lysosomes. We further demonstrate that human and Xenopus oocytes have a Balbiani body characterized by a dense accumulation of mitochondria in their cytoplasm. However, despite previous reports, we did not find a Balbiani body in mouse oocytes. Instead, we demonstrate that what was previously used as a marker for the Balbiani body in mouse primordial oocytes is in fact a ring-shaped Golgi that is not functionally associated with oocyte dormancy. This study provides the first insights into the organization of the cytoplasm in mammalian primordial oocytes, and clarifies the relative advantages and limitations of choosing different model organisms for studying oocyte dormancy.
- dc.description.sponsorship This study was supported by a European Research Council Starting Grant (ERC-StG-2017-759107) and the Ministerio de Ciencia e Innovación (MINECO - BFU2017-89373-P and PID2020-115127GB-I00 to E.B.). G.Z. acknowledges funding from the European Union's Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 754422. M.S. is supported by a Juan de la Cierva-Formación fellowship from the Ministerio de Ciencia e Innovación (FJC2019-041607-I; AEI; 10.13039/501100011033).
- dc.format.mimetype application/pdf
- dc.identifier.citation Dhandapani L, Salzer MC, Duran JM, Zaffagnini G, De Guirior C, Martínez-Zamora MA, Böke E. Comparative analysis of vertebrates reveals that mouse primordial oocytes do not contain a Balbiani body. J Cell Sci. 2022;135(1):jcs259394. DOI: 10.1242/jcs.259394
- dc.identifier.doi http://dx.doi.org/10.1242/jcs.259394
- dc.identifier.issn 0021-9533
- dc.identifier.uri http://hdl.handle.net/10230/52200
- dc.language.iso eng
- dc.publisher Company of Biologists
- dc.relation.ispartof J Cell Sci. 2022;135(1):jcs259394
- dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/759107
- dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/BFU2017-89373-P
- dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PID2020-115127GB-I00
- dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/754422
- dc.rights © Company of Biologists https://doi.org/10.1242/jcs.259394 DOI: 10.1242/jcs.259394
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.subject.keyword Dormancy
- dc.subject.keyword Fertility
- dc.subject.keyword Primordial oocyte
- dc.subject.keyword The Balbiani body
- dc.title Comparative analysis of vertebrates reveals that mouse primordial oocytes do not contain a Balbiani body
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion