The conformation of yeast chromosome III is mating type dependent and controlled by the recombination enhancer

Belton, Jon-Matthew; Lajoie, Bryan R.; Audibert, Sylvain; Cantaloube, Sylvain; Lassadi, Imen; Goiffon, Isabelle; Baù, Davide; Marti-Renom, Marc A.; Bystricky, Kerstin; Dekker, Job

The conformation of yeast chromosome III is mating type dependent and controlled by the recombination enhancer

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dc.contributor.author Belton, Jon-Matthew
dc.contributor.author Lajoie, Bryan R.
dc.contributor.author Audibert, Sylvain
dc.contributor.author Cantaloube, Sylvain
dc.contributor.author Lassadi, Imen
dc.contributor.author Goiffon, Isabelle
dc.contributor.author Baù, Davide
dc.contributor.author Marti-Renom, Marc A.
dc.contributor.author Bystricky, Kerstin
dc.contributor.author Dekker, Job
dc.date.accessioned 2023-06-27T06:18:56Z
dc.date.available 2023-06-27T06:18:56Z
dc.date.issued 2015
dc.description.abstract Mating-type switching in yeast occurs through gene conversion between the MAT locus and one of two silent loci (HML or HMR) on opposite ends of the chromosome. MATa cells choose HML as template, whereas MATα cells use HMR. The recombination enhancer (RE) located on the left arm regulates this process. One long-standing hypothesis is that switching is guided by mating-type-specific and possibly RE-dependent chromosome folding. Here, we use Hi-C, 5C, and live-cell imaging to characterize the conformation of chromosome III in both mating types. We discovered a mating-type-specific conformational difference in the left arm. Deletion of a 1-kb subregion within the RE, which is not necessary during switching, abolished mating-type-dependent chromosome folding. The RE is therefore a composite element with one subregion essential for donor selection during switching and a separate region involved in modulating chromosome conformation.
dc.description.sponsorship We acknowledge financial support from the National Human Genome Research Institute (HG003143) to J.D.; the Human Frontiers Science Program (RGP0044/2011) to J.D., K.B., and M.A.M.-R.; the Spanish MINECO (BFU2010-19310/BMC and BFU2013-47736-P) to M.A.M.-R.; and the French ANR (SVSE5 ANDY) to K.B. J.D. is an investigator of the Howard Hughes Medical Institute.
dc.format.mimetype application/pdf
dc.identifier.citation Belton JM, Lajoie BR, Audibert S, Cantaloube S, Lassadi I, Goiffon I, et al. The conformation of yeast chromosome III is mating type dependent and controlled by the recombination enhancer. Cell Reports. 2015 Dec 01;13(9):1855-67. DOI: 10.1016/j.celrep.2015.10.063
dc.identifier.doi http://dx.doi.org/10.1016/j.celrep.2015.10.063
dc.identifier.issn 2211-1247
dc.identifier.uri http://hdl.handle.net/10230/57356
dc.language.iso eng
dc.publisher Cell Press
dc.relation.ispartof Cell Reports. 2015 Dec 01;13(9):1855-67
dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2013-47736-P
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2010-19310/BMC
dc.rights This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.keyword Chromosome conformation
dc.subject.keyword Mating-type switching
dc.subject.keyword Long-range interactions
dc.subject.keyword Recombination enhancer
dc.subject.keyword Multicolor fluorescence microscopy
dc.title The conformation of yeast chromosome III is mating type dependent and controlled by the recombination enhancer
dc.type info:eu-repo/semantics/article
dc.type.version info:eu-repo/semantics/publishedVersion

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