Differential structural features in soleus and gastrocnemius of carnitine-treated cancer cachectic rats
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- dc.contributor.author Busquets, Silvia
- dc.contributor.author Pérez-Peiró, Maria
- dc.contributor.author Salazar Degracia, Anna
- dc.contributor.author Argilés, Josep M.
- dc.contributor.author Serpe, Roberto
- dc.contributor.author Rojano-Toimil, Alba
- dc.contributor.author López Soriano, Francisco J.
- dc.contributor.author Barreiro Portela, Esther
- dc.date.accessioned 2020-02-06T07:26:54Z
- dc.date.issued 2020
- dc.description.abstract Muscle wasting is associated with chronic diseases and cancer. Elucidation of the biological mechanism involved in the process of muscle mass loss and cachexia may help identify therapeutic targets. We hypothesized that l-carnitine treatment may differentially revert muscle fiber atrophy and other structural alterations in slow- and fast-twitch limb muscles of rats bearing the Yoshida ascites hepatoma. In soleus and gastrocnemius of tumor-bearing rats (108 AH-130 Yoshida ascites hepatoma cells inoculated intraperitoneally) with and without treatment with l-carnitine (1 g/kg body weight for 7 days, intragastric), food intake, body and muscle weights, fiber typing and morphometry, morphological features, redox balance, autophagy and proteolytic, and signaling markers were explored. Levels of carnitine palmitoyl transferase were also measured in all the study muscles. l-Carnitine treatment ameliorated the atrophy of both slow- and fast-twitch fibers (gastrocnemius particularly), muscle structural alterations (both muscles), and attenuated oxidative stress, proteolytic and signaling markers (gastrocnemius). Despite that carnitine palmitoyl transferase-1 levels increased in both muscle types in a similar fashion, l-carnitine ameliorated muscle atrophy and proteolysis in a muscle-specific manner in cancer-induced cachexia. These data reveal the need to study muscles of different fiber type composition and function to better understand whereby l-carnitine exerts its beneficial effects on the myofibers in muscle wasting processes. These findings also have potential clinical implications, since combinations of various exercise and muscle training modalities with l-carnitine should be specifically targeted for the muscle groups to be trained.
- dc.description.sponsorship The current research has been supported by Instituto de Salud Carlos‐III, contract grant numbers, CIBERES, FIS 14/00713 (FEDER), FIS 18/00075 (FEDER), Spanish Ministry of Science and Innovation, contract grant number SAF 2011–26091, Spanish Respiratory Society (SEPAR), contract grant numbers, SEPAR 2016, SEPAR 2018, Catalan Foundation of Pulmonology (FUCAP), contract grant numbers, FUCAP 2016.
- dc.format.mimetype application/pdf
- dc.identifier.citation Busquets S, Pérez-Peiró M, Salazar-Degracia A, Argilés JM, Serpe R, Rojano-Toimil A. et al. Differential structural features in soleus and gastrocnemius of carnitine-treated cancer cachectic rats. J Cell Physiol. 2020 Jan;235(1):526-37. DOI: 10.1002/jcp.28992
- dc.identifier.doi http://dx.doi.org/10.1002/jcp.28992
- dc.identifier.issn 0021-9541
- dc.identifier.uri http://hdl.handle.net/10230/43499
- dc.language.iso eng
- dc.publisher Wiley
- dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/SAF2011‐26091
- dc.rights This is the peer reviewed version of the following article: Busquets S, Pérez-Peiró M, Salazar-Degracia A, Argilés JM, Serpe R, Rojano-Toimil A. et al. Differential structural features in soleus and gastrocnemius of carnitine-treated cancer cachectic rats. J Cell Physiol. 2020 Jan;235(1):526-537, which has been published in final form at http://dx.doi.org/10.1002/jcp.28992. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.subject.keyword Experimental cancer-induced cachexia
- dc.subject.keyword l-carnitine
- dc.subject.keyword Muscle fiber type and morphometry
- dc.subject.keyword Muscle structure and morphology
- dc.subject.keyword Proteolytic
- dc.subject.keyword Autophagy, and signaling markers
- dc.subject.keyword slow- and fast-twitch muscles
- dc.title Differential structural features in soleus and gastrocnemius of carnitine-treated cancer cachectic rats
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/acceptedVersion