Epitope characterization of a monoclonal antibody that selectively recognizes KIR2DL1 allotypes

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  • dc.contributor.author Falco, Michela
  • dc.contributor.author Meazza, Raffaella
  • dc.contributor.author Alicata, Claudia
  • dc.contributor.author Canevali, Paolo
  • dc.contributor.author Muntasell i Castellví, Aura, 1972-
  • dc.contributor.author Bottino, Cristina
  • dc.contributor.author Moretta, Lorenzo
  • dc.contributor.author Pende, Daniela
  • dc.contributor.author López-Botet, M. (Miguel)
  • dc.date.accessioned 2022-09-21T06:13:10Z
  • dc.date.available 2022-09-21T06:13:10Z
  • dc.date.issued 2022
  • dc.description.abstract Killer immunoglobulin-like receptor (KIR) genes code for a family of inhibitory and activating receptors, finely tuning NK cell function. Numerous studies reported the relevance of KIR allelic polymorphism on KIR expression, ligand affinity, and strength in signal transduction. Although KIR variability, including gene copy number and allelic polymorphism, in combination with HLA class I polymorphism, impacts both KIR expression and NK cell education, only a precise phenotypic analysis can define the size of the different KIRpos NK cell subsets. In this context, reagents recognizing a limited number of KIRs is essential. In this study, we have characterized the specificity of an anti-KIR mAb termed HP-DM1. Testing its binding to HEK-293T cells transfected with plasmids coding for different KIRs, we demonstrated that HP-DM1 mAb exclusively reacts with KIR2DL1. Using site-directed mutagenesis, we identified the four amino acids relevant for HP-DM1 recognition: M44, S67, R68, and T70. HP-DM1 mAb binds to a conformational epitope including M44, the residue crucial for HLA-C K80 recognition by KIR2DL1. Based on the HP-DM1 epitope characterization, we could extend its reactivity to all KIR2DL1 allotypes identified except for KIR2DL1*022 and, most likely, KIR2DL1*020, predicting that it does not recognize any other KIR with the only exception of KIR2DS1*013. Moreover, by identifying the residues relevant for HP-DM1 binding, continuously updating of its reactivity will be facilitated.
  • dc.description.sponsorship This work was supported by Italian Ministry of Health (Ricerca Corrente G. Gaslini, Ricerca Corrente Ospedale Policlinico San Martino) (Cristina Bottino and Daniela Pende), Instituto de Salud Carlos III, FIS00/0181 (to Miguel Lopez-Botet), H2020-MSCA-ITN-2017-765104-MATURE-NK (to Miguel Lopez-Botet and Daniela Pende), AIRC 5×1000 2018 id. 21147 (Lorenzo Moretta).
  • dc.format.mimetype application/pdf
  • dc.identifier.citation Falco M, Meazza R, Alicata C, Canevali P, Muntasell A, Bottino C, Moretta L, Pende D, Lopez-Botet M. Epitope characterization of a monoclonal antibody that selectively recognizes KIR2DL1 allotypes. HLA. 2022 Aug;100(2):107-18. DOI: 10.1111/tan.14630
  • dc.identifier.doi http://dx.doi.org/10.1111/tan.14630
  • dc.identifier.issn 2059-2302
  • dc.identifier.uri http://hdl.handle.net/10230/54129
  • dc.language.iso eng
  • dc.publisher Wiley
  • dc.relation.ispartof HLA. 2022 Aug;100(2):107-18
  • dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/765104
  • dc.rights © 2022 The Authors. HLA: Immune Response Genetics published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/
  • dc.subject.keyword Killer immunoglobulin-like receptors
  • dc.subject.keyword Monoclonal antibodies
  • dc.subject.keyword Natural killer cells
  • dc.subject.keyword Polymorphism
  • dc.subject.keyword Site-directed mutagenesis
  • dc.title Epitope characterization of a monoclonal antibody that selectively recognizes KIR2DL1 allotypes
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/publishedVersion

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