Ten steps to get started in Genome Assembly and Annotation

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• dc.contributor.author Dominguez Del Angel, Victoria
• dc.contributor.author Hjerde, Erik
• dc.contributor.author Sterck, Lieven
• dc.contributor.author Capella Gutiérrez, Salvador Jesús, 1985-
• dc.contributor.author Notredame, Cedric
• dc.contributor.author Vinnere Pettersson, Olga
• dc.contributor.author Amselem, Joëlle
• dc.contributor.author Bouri, Laurent
• dc.contributor.author Bocs, Stephanie
• dc.contributor.author Klopp, Christophe
• dc.contributor.author Gibrat, Jean-François
• dc.contributor.author Vlasova, Anna
• dc.contributor.author Leskosek, Brane L.
• dc.contributor.author Soler, Lucile
• dc.contributor.author Binzer-Panchai, Mahesh
• dc.contributor.author Lantz, Henrik
• dc.date.accessioned 2019-11-15T08:12:51Z
• dc.date.available 2019-11-15T08:12:51Z
• dc.date.issued 2018
• dc.description.abstract As a part of the ELIXIR-EXCELERATE efforts in capacity building, we present here 10 steps to facilitate researchers getting started in genome assembly and genome annotation. The guidelines given are broadly applicable, intended to be stable over time, and cover all aspects from start to finish of a general assembly and annotation project. Intrinsic properties of genomes are discussed, as is the importance of using high quality DNA. Different sequencing technologies and generally applicable workflows for genome assembly are also detailed. We cover structural and functional annotation and encourage readers to also annotate transposable elements, something that is often omitted from annotation workflows. The importance of data management is stressed, and we give advice on where to submit data and how to make your results Findable, Accessible, Interoperable, and Reusable (FAIR).
• dc.description.sponsorship ELIXIR-EXCELERATE is funded by the European Commission within the Research Infrastructures Programme of Horizon 2020 [676559].
• dc.format.mimetype application/pdf
• dc.identifier.citation Dominguez Del Angel V, Hjerde E, Sterck L, Capella-Gutierrez S, Notredame C, Vinnere Pettersson O et al. Ten steps to get started in Genome Assembly and Annotation. F1000Res. 2018;7(ELIXIR):148. DOI: 10.12688/f1000research.13598.1
• dc.identifier.doi http://dx.doi.org/10.12688/f1000research.13598.1
• dc.identifier.issn 2046-1402
• dc.identifier.uri http://hdl.handle.net/10230/42860
• dc.language.iso eng
• dc.publisher F1000Research
• dc.relation.ispartof F1000Res. 2018;7(ELIXIR):148
• dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/676559
• dc.rights © 2018 Dominguez Del Angel V et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Genome
• dc.subject.keyword Assembly
• dc.subject.keyword Annotation
• dc.subject.keyword FAIR
• dc.subject.keyword NGS
• dc.subject.keyword Workflows
• dc.subject.keyword DNA
• dc.title Ten steps to get started in Genome Assembly and Annotation
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion