Transcriptomic effects of tributyltin (TBT) in zebrafish eleutheroembryos. A functional benchmark dose analysis

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• dc.contributor.author Martínez, Rubén
• dc.contributor.author Esteve-Codina, Anna
• dc.contributor.author Barata, Carlos
• dc.contributor.author Tauler, Romà
• dc.contributor.author Piña, Benjamin
• dc.contributor.author Navarro-Martín, Laia
• dc.date.accessioned 2023-10-05T06:06:45Z
• dc.date.available 2023-10-05T06:06:45Z
• dc.date.issued 2020
• dc.description.abstract Exposure to the antifouling tributyltin (TBT) has been related to imposex in mollusks and to obesogenicity, adipogenesis and masculinization in fish. To understand the underlying molecular mechanisms, we evaluated dose-response effects of TBT (1.7-56 nM) in zebrafish eleutheroembryos transcriptome exposed from 2 to 5 days post-fertilization. RNA-sequencing analysis identified 3238 differentially expressed transcripts in eleutheroembryos exposed to TBT. Benchmark dose analyses (BMD) showed that the point of departure (PoD) for transcriptomic effects (9.28 nM) was similar to the metabolomic PoD (11.5 nM) and about one order of magnitude lower than the morphometric PoD (67.9 nM) or the median lethal concentration (LC50: 93.6 nM). Functional analysis of BMD transcriptomic data identified steroid metabolism and cholesterol and vitamin D3 biosynthesis as the most sensitive pathways to TBT (<50% PoD). Conversely, transcripts related to general stress and DNA damage became affected only at doses above the PoD. Therefore, our results indicate that transcriptomes can act as early molecular indicators of pollutant exposure, and illustrates their usefulness for the mechanistic identification of the initial toxic events. As the estimated molecular PoDs are close to environmental levels, we concluded that TBT may represent a substantial risk in some natural environments.
• dc.description.sponsorship This work was supported by the European Research Council under the European Union's Seventh Framework Programme (FP/ 2007-2013)/ERC Grant Agreement n. 320737. Some part of this study was also supported by a grant from the Spanish Ministry of Economy and Competitiveness (CTQ2014-56777-R). AEC acknowledges the funding from ISCIII (Spanish Ministry of Economy and Competitiveness, grant number PT17/0009/0019), co-financed by the European Fund for Regional Development (FEDER). RM was supported by a FPU predoctoral fellow from the Spanish Ministry of Education, Culture and Sport (ref. FPU15/03332).
• dc.format.mimetype application/pdf
• dc.identifier.citation Martínez R, Esteve-Codina A, Barata C, Tauler R, Piña B, Navarro-Martín L. Transcriptomic effects of tributyltin (TBT) in zebrafish eleutheroembryos. A functional benchmark dose analysis. J Hazard Mater. 2020 Nov 5;398:122881. DOI: 10.1016/j.jhazmat.2020.122881
• dc.identifier.doi http://dx.doi.org/10.1016/j.jhazmat.2020.122881
• dc.identifier.issn 1873-3336
• dc.identifier.uri http://hdl.handle.net/10230/58046
• dc.language.iso eng
• dc.publisher Elsevier
• dc.relation.ispartof J Hazard Mater. 2020 Nov 5;398:122881
• dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/320737
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/CTQ2014-56777-R
• dc.rights © 2020 Published by Elsevier B.V. http://dx.doi.org/10.1016/j.jhazmat.2020.122881
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.subject.keyword Benchmark dose
• dc.subject.keyword Endocrine disruption
• dc.subject.keyword RNA-Seq
• dc.subject.keyword Tributyltin
• dc.subject.keyword Zebrafish
• dc.title Transcriptomic effects of tributyltin (TBT) in zebrafish eleutheroembryos. A functional benchmark dose analysis
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/acceptedVersion