Tungstate-targeting of BKαβ1 channels tunes ERK phosphorylation and cell proliferation in human vascular smooth muscle

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  • dc.contributor.author Fernández Mariño, Ana Isabel, 1984-ca
  • dc.contributor.author Cidad, Pilarca
  • dc.contributor.author Zafra, Deliaca
  • dc.contributor.author Nocito, Lauraca
  • dc.contributor.author Domínguez, Jorgeca
  • dc.contributor.author Oliván Viguera, Aidaca
  • dc.contributor.author Köhler, Ralfca
  • dc.contributor.author López López, José R.ca
  • dc.contributor.author Pérez García, María Teresaca
  • dc.contributor.author Valverde, M. A. (Miguel Ángel), 1963-ca
  • dc.contributor.author Guinovart, Joan J. (Joan Josep), 1947-ca
  • dc.contributor.author Fernández-Fernández, José Manuel, 1967-ca
  • dc.date.accessioned 2016-01-21T13:28:27Z
  • dc.date.available 2016-01-21T13:28:27Z
  • dc.date.issued 2015
  • dc.description.abstract Despite the substantial knowledge on the antidiabetic, antiobesity and antihypertensive actions of tungstate, information on its primary target/s is scarce. Tungstate activates both the ERK1/2 pathway and the vascular voltage- and Ca2+-dependent large-conductance BKαβ1 potassium channel, which modulates vascular smooth muscle cell (VSMC) proliferation and function, respectively. Here, we have assessed the possible involvement of BKαβ1 channels in the tungstate-induced ERK phosphorylation and its relevance for VSMC proliferation. Western blot analysis in HEK cell lines showed that expression of vascular BKαβ1 channels potentiates the tungstate-induced ERK1/2 phosphorylation in a Gi/o protein-dependent manner. Tungstate activated BKαβ1 channels upstream of G proteins as channel activation was not altered by the inhibition of G proteins with GDPβS or pertussis toxin. Moreover, analysis of Gi/o protein activation measuring the FRET among heterologously expressed Gi protein subunits suggested that tungstate-targeting of BKαβ1 channels promotes G protein activation. Single channel recordings on VSMCs from wild-type and β1-knockout mice indicated that the presence of the regulatory β1 subunit was essential for the tungstate-mediated activation of BK channels in VSMCs. Moreover, the specific BK channel blocker iberiotoxin lowered tungstate-induced ERK phosphorylation by 55% and partially reverted (by 51%) the tungstate-produced reduction of platelet-derived growth factor (PDGF)-induced proliferation in human VSMCs. Our observations indicate that tungstate-targeting of BKαβ1 channels promotes activation of PTX-sensitive Gi proteins to enhance the tungstate-induced phosphorylation of ERK, and inhibits PDGF-stimulated cell proliferation in human vascular smooth muscle.ca
  • dc.description.sponsorship This work was supported by grants from the Spanish Ministry of Economy and Competitiveness (SAF2012-31089 to JMFF, SAF2012-38140 to MAV, BFU2013-45867-R to JRLL), FEDER Funds, Ministry of Science and Innovation (BFU 2008-00769 to JG, BFU2010-15898 to MTPG), Instituto de Salud Carlos III (RIC RD12/0042/0006, RD12/0042/0014, Red HERACLES) and Junta de Castilla y León (VA094A11-2 to JRLL) and European Community (FP7-People-CIG-321721 to RK).
  • dc.format.mimetype application/pdfca
  • dc.identifier.citation Fernández-Mariño AI, Cidad P, Zafra D, Nocito L, Domínguez J, Oliván-Viguera A et al. Tungstate-targeting of BKαβ1 channels tunes ERK phosphorylation and cell proliferation in human vascular smooth muscle. PLoS One. 2015; 10(2): e0118148. DOI 10.1371/journal.pone.0118148ca
  • dc.identifier.doi http://dx.doi.org/10.1371/journal.pone.0118148
  • dc.identifier.issn 1932-6203
  • dc.identifier.uri http://hdl.handle.net/10230/25625
  • dc.language.iso engca
  • dc.publisher Public Library of Science (PLoS)ca
  • dc.relation.ispartof PLoS One. 2015;10(2):e0118148
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/SAF2012-31089
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/SAF2012-38140
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2013-45867
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2008-00769
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2010-15898
  • dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/321721
  • dc.rights © 1969 Fernández-Mariño et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are creditedca
  • dc.rights.accessRights info:eu-repo/semantics/openAccessca
  • dc.rights.uri http://creativecommons.org/licenses/by/4.0/ca
  • dc.subject.other Calci -- Metabolismeca
  • dc.subject.other Ratolins (Animals de laboratori)ca
  • dc.title Tungstate-targeting of BKαβ1 channels tunes ERK phosphorylation and cell proliferation in human vascular smooth muscleca
  • dc.type info:eu-repo/semantics/articleca
  • dc.type.version info:eu-repo/semantics/publishedVersionca

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