Gradual compaction of the central spindle decreases its dynamicity in PRC1 and EB1 gene-edited cells

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  • dc.contributor.author Asthana, Jayant
  • dc.contributor.author Cade, Nicholas Ian
  • dc.contributor.author Normanno, Davide
  • dc.contributor.author Lim, Wei Ming
  • dc.contributor.author Surrey, Thomas
  • dc.date.accessioned 2022-01-25T11:46:32Z
  • dc.date.available 2022-01-25T11:46:32Z
  • dc.date.issued 2021
  • dc.description.abstract During mitosis, the spindle undergoes morphological and dynamic changes. It reorganizes at the onset of the anaphase when the antiparallel bundler PRC1 accumulates and recruits central spindle proteins to the midzone. Little is known about how the dynamic properties of the central spindle change during its morphological changes in human cells. Using gene editing, we generated human cells that express from their endogenous locus fluorescent PRC1 and EB1 to quantify their native spindle distribution and binding/unbinding turnover. EB1 plus end tracking revealed a general slowdown of microtubule growth, whereas PRC1, similar to its yeast orthologue Ase1, binds increasingly strongly to compacting antiparallel microtubule overlaps. KIF4A and CLASP1 bind more dynamically to the central spindle, but also show slowing down turnover. These results show that the central spindle gradually becomes more stable during mitosis, in agreement with a recent "bundling, sliding, and compaction" model of antiparallel midzone bundle formation in the central spindle during late mitosis.
  • dc.description.sponsorship This work was supported by the Francis Crick Institute, which receives its core funding from Cancer Research UK (FC001163), the UK Medical Research Council (FC001163), and the Wellcome Trust (FC001163). T Surrey acknowledges support from the European Research Council (Advanced Grant, project 323042). J Asthana, D Normanno, WM Lim, and T Surrey acknowledge also the support of the Spanish Ministry of Economy, Industry and Competitiveness to the CRG-EMBL partnership, the Centro de Excelencia Severo Ochoa and the CERCA Programme of the Generalitat de Catalunya
  • dc.format.mimetype application/pdf
  • dc.identifier.citation Asthana J, Cade NI, Normanno D, Lim WM, Surrey T. Gradual compaction of the central spindle decreases its dynamicity in PRC1 and EB1 gene-edited cells. Life Sci Alliance. 2021 Sep 27;4(12):e202101222. DOI: 10.26508/lsa.202101222
  • dc.identifier.doi http://dx.doi.org/10.26508/lsa.202101222
  • dc.identifier.issn 2575-1077
  • dc.identifier.uri http://hdl.handle.net/10230/52314
  • dc.language.iso eng
  • dc.publisher Life Science Alliance
  • dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/323042
  • dc.rights © 2021 Asthana et al. This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0)
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.rights.uri https://creativecommons.org/licenses/by/4.0/
  • dc.subject.other Genètica
  • dc.subject.other Mitosi
  • dc.subject.other Proteïnes
  • dc.title Gradual compaction of the central spindle decreases its dynamicity in PRC1 and EB1 gene-edited cells
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/publishedVersion

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