LSm1-7 complexes bind to specific sites in viral RNA genomes and regulate their translation and replication

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• dc.contributor.author Galão, Rui Pedro Ribeiroca
• dc.contributor.author Chari, Ashwinca
• dc.contributor.author Alves-Rodrigues, Isabelca
• dc.contributor.author Lobão, Danielaca
• dc.contributor.author Mas, Antonio Juanca
• dc.contributor.author Kambach, Christianca
• dc.contributor.author Fischer, Utzca
• dc.contributor.author Díez Antón, Juana, 1962-ca
• dc.date.accessioned 2016-01-19T13:30:51Z
• dc.date.available 2016-01-19T13:30:51Z
• dc.date.issued 2010
• dc.description.abstract LSm1-7 complexes promote cellular mRNA degradation, in addition to translation and replication of positive-strand RNA viruses such as the Brome mosaic virus (BMV). Yet, how LSm1-7 complexes act on their targets remains elusive. Here, we report that reconstituted recombinant LSm1-7 complexes directly bind to two distinct RNA-target sequences in the BMV genome, a tRNA-like structure at the 3'-untranslated region and two internal A-rich single-stranded regions. Importantly, in vivo analysis shows that these sequences regulate the translation and replication of the BMV genome. Furthermore, both RNA-target sequences resemble those found for Hfq, the LSm counterpart in bacteria, suggesting conservation through evolution. Our results provide the first evidence that LSm1-7 complexes interact directly with viral RNA genomes and open new perspectives in the understanding of LSm1-7 functions.ca
• dc.description.sponsorship This work was supported by grants from the Spanish Ministerio de Educación y Ciencia (BFU2007-66933/BMC) and the German Research Foundation (DFG-FOR855). I.A.-R. and D.L. were supported by Fundaçao para a Ciência e Tecnología (SARH/BD/9630/2002; SFRH/BD/37047/2007)
• dc.format.mimetype application/pdfca
• dc.identifier.citation Galão RP, Chari A, Alves-Rodrigues I, Lobão D, Mas A, Kambach C et al. LSm1-7 complexes bind to specific sites in viral RNA genomes and regulate their translation and replication. RNA. 2010;16(4):817-27. DOI: 10.1261/rna.1712910ca
• dc.identifier.doi http://dx.doi.org/10.1261/rna.1712910
• dc.identifier.issn 1355-8382
• dc.identifier.uri http://hdl.handle.net/10230/25602
• dc.language.iso engca
• dc.publisher Cold Spring Harbor Laboratory Press (CSHL Press)ca
• dc.relation.ispartof RNA. 2010;16(4):817-27
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PN/BFU2007-66933
• dc.rights Copyright © 2010, RNA by RNA Society. Published version available at http://rnajournal.cshlp.org/content/16/4/817.longca
• dc.rights.accessRights info:eu-repo/semantics/openAccessca
• dc.subject.keyword BMV
• dc.subject.keyword LSm1-7
• dc.subject.keyword RNA virus
• dc.subject.keyword Sm proteins
• dc.subject.keyword Translation
• dc.subject.keyword Replication
• dc.subject.other Interaccions RNA-proteïnaca
• dc.subject.other Saccharomyces cerevisiae -- Metabolismeca
• dc.title LSm1-7 complexes bind to specific sites in viral RNA genomes and regulate their translation and replicationca
• dc.type info:eu-repo/semantics/articleca
• dc.type.version info:eu-repo/semantics/publishedVersionca