Distinct roles of the polarity factors Boi1 and Boi2 in the control of exocytosis and abscission in budding yeast

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  • dc.contributor.author Masgrau Fortuny, Aina, 1986-
  • dc.contributor.author Battola, Andrea, 1987-
  • dc.contributor.author Sanmartín Olmo, Trinidad
  • dc.contributor.author Pryszcz, Leszek Piotr, 1985-
  • dc.contributor.author Gabaldón Estevan, Juan Antonio, 1973-
  • dc.contributor.author Mendoza, Manuel (Mendoza Palomares)
  • dc.date.accessioned 2024-02-26T07:23:10Z
  • dc.date.available 2024-02-26T07:23:10Z
  • dc.date.issued 2017
  • dc.description.abstract Boi1 and Boi2 (Boi1/2) are budding yeast plasma membrane proteins that function in polarized growth, and in cytokinesis inhibition in response to chromosome bridges via the NoCut abscission checkpoint. How Boi1/2 act in these two distinct processes is not understood. We demonstrate that Boi1/2 are required for a late step in the fusion of secretory vesicles with the plasma membrane of the growing bud. Cells lacking Boi1/2 accumulate secretory vesicles and are defective in bud growth. In contrast, Boi2 is specifically required for abscission inhibition in cells with chromatin bridges. The SH3 domain of Boi2, which is dispensable for bud growth and targets Boi2 to the site of abscission, is necessary and sufficient for abscission inhibition. Gain of function of the exocyst, a conserved protein complex involved in tethering of exocytic vesicles to the plasma membrane, rescued secretion and bud growth defects in boi mutant cells, and abrogated NoCut checkpoint function. Thus Boi2 functions redundantly with Boi1 to promote the fusion of secretory vesicles with the plasma membrane at sites of polarized growth, and acts as an abscission inhibitor during cytokinesis in response to chromatin bridges.
  • dc.description.sponsorship We are grateful to Karolina Jodkowska for assistance with the initial characterization of EXO70*. We thank Yves Barral, Amy Curwin, Vivek Malhotra, Snezhana Oliferenko, Isabelle Sagot, Jerome Solon, and members of the Mendoza laboratory for helpful suggestions; Marti Aldea, Eric Bailly, Derek McCusker, David Pellman, and Randy Schekman for providing reagents; the CRG Genomics and Advanced Light Microscopy Units; and the CCiT Electron Cryomicroscopy Unit (Universitat de Barcelona). This research is supported by grants from the Spanish Ministry of Economy and Competitiveness (BFU09-08213 and BFU2012-37162 to M.M. and BIO2012-37161 to T.G.), the Qatar National Research Fund (NPRP 5-298-3-086 to T.G.), and the European Research Council (ERC Starting Grant 260965 to M.M. and 310325 to T.G.). L.P.P. was funded through La Caixa–CRG International Fellowship Program.
  • dc.format.mimetype application/pdf
  • dc.identifier.citation Masgrau A, Battola A, Sanmartin T, Pryszcz LP, Gabaldón T, Mendoza M. Distinct roles of the polarity factors Boi1 and Boi2 in the control of exocytosis and abscission in budding yeast. MBoC. 2017 Nov 01;28(22):3082-94. DOI: 10.1091/mbc.e17-06-0404
  • dc.identifier.doi http://dx.doi.org/10.1091/mbc.e17-06-0404
  • dc.identifier.issn 1939-4586
  • dc.identifier.uri http://hdl.handle.net/10230/59255
  • dc.language.iso eng
  • dc.publisher American Society for Cell Biology
  • dc.relation.ispartof Molecular Biology of the Cell. 2017 Nov 01;28(22):3082-94
  • dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/260965
  • dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/310325
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU09-08213
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2012-37162
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BIO2012-37161
  • dc.rights © 2017 Masgrau, Battola et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.rights.uri http://creativecommons.org/licenses/by-nc-sa/3.0
  • dc.subject.other Citocinesi
  • dc.subject.other Cèl·lules
  • dc.subject.other Cromatina
  • dc.title Distinct roles of the polarity factors Boi1 and Boi2 in the control of exocytosis and abscission in budding yeast
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/publishedVersion

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