A novel type of light-harvesting antenna protein of red algal origin in algae with secondary plastids

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• dc.contributor.author Sturm, Sabineca
• dc.contributor.author Engelken, Johannesca
• dc.contributor.author Gruber, Ansgarca
• dc.contributor.author Vugrinec, Saschaca
• dc.contributor.author Kroth, Peter Gca
• dc.contributor.author Adamska, Iwonaca
• dc.contributor.author Lavaud, Johannca
• dc.date.accessioned 2015-03-18T09:11:03Z
• dc.date.available 2015-03-18T09:11:03Z
• dc.date.issued 2013ca
• dc.description.abstract Background: Light, the driving force of photosynthesis, can be harmful when present in excess; therefore, any light harvesting system requires photoprotection. Members of the extended light-harvesting complex (LHC) protein superfamily are involved in light harvesting as well as in photoprotection and are found in the red and green plant lineages, with a complex distribution pattern of subfamilies in the different algal lineages. Results: Here, we demonstrate that the recently discovered “red lineage chlorophyll a/b-binding-like proteins” (RedCAPs) form a monophyletic family within this protein superfamily. The occurrence of RedCAPs was found to be restricted to the red algal lineage, including red algae (with primary plastids) as well as cryptophytes, haptophytes and heterokontophytes (with secondary plastids of red algal origin). Expression of a full-length RedCAP:GFP fusion construct in the diatom Phaeodactylum tricornutum confirmed the predicted plastid localisation of RedCAPs. Furthermore, we observed that similarly to the fucoxanthin chlorophyll a/c-binding light-harvesting antenna proteins also RedCAP transcripts in diatoms were regulated in a diurnal way at standard light conditions and strongly repressed at high light intensities./nConclusions: The absence of RedCAPs from the green lineage implies that RedCAPs evolved in the red lineage after separation from the the green lineage. During the evolution of secondary plastids, RedCAP genes therefore must have been transferred from the nucleus of the endocytobiotic alga to the nucleus of the host cell, a process that involved complementation with pre-sequences allowing import of the gene product into the secondary plastid bound by four membranes. Based on light-dependent transcription and on localisation data, we propose that RedCAPs might participate in the light (intensity and quality)-dependent structural or functional reorganisation of the light-harvesting antennae of the photosystems upon dark to light shifts as regularly experienced by diatoms in nature. Remarkably, in plastids of the red lineage as well as in green lineage plastids, the phycobilisome based cyanobacterial light harvesting system has been replaced by light harvesting systems that are based on members of the extended LHC protein superfamily, either for one of the photosystems (PS I of red algae) or for both (diatoms). In their proposed function, the RedCAP protein family may thus have played a role in the evolutionary structural remodelling of light-harvesting antennae in the red lineage.en
• dc.description.sponsorship This work was supported by the Universität Konstanz and by grants of the Deutsche Forschungsgemeinschaft (KR 1661/3 to PGK, AD 92/7-2 to IA, LA 2368/2-1 to JL). JE was supported by a grant (I/82 750) from the Volkswagenstiftung (“Förderungsinitiative Evolutionsbiologie”)en
• dc.format.mimetype application/pdfca
• dc.identifier.citation Sturm S, Engelken J, Gruber A, Vugrinec S, Kroth PG, Adamska I, Lavaud J. A novel type of light-harvesting antenna protein of red algal origin in algae with secondary plastids. BMC Evolutionary Biology. 2013; 13: 159. DOI 10.1186/1471-2148-13-159ca
• dc.identifier.doi http://dx.doi.org/10.1186/1471-2148-13-159
• dc.identifier.issn 1471-2148ca
• dc.identifier.uri http://hdl.handle.net/10230/23211
• dc.language.iso engca
• dc.publisher BioMed Centralca
• dc.relation.ispartof BMC Evolutionary Biology. 2013; 13: 159
• dc.rights © 2013 Sturm et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.ca
• dc.rights.accessRights info:eu-repo/semantics/openAccessca
• dc.rights.uri http://creativecommons.org/licenses/by/2.0
• dc.subject.keyword Complex plastidsen
• dc.subject.keyword Diatomsen
• dc.subject.keyword Chloroplasten
• dc.subject.keyword Gene transferen
• dc.subject.keyword Light-harvesting antenna proteinsen
• dc.subject.keyword Red lineage chlorophyll a/b-binding-like proteinsen
• dc.subject.other Seqüència d'aminoàcidsca
• dc.subject.other Proteïnes -- Metabolismeca
• dc.title A novel type of light-harvesting antenna protein of red algal origin in algae with secondary plastidsen
• dc.type info:eu-repo/semantics/articleca
• dc.type.version info:eu-repo/semantics/publishedVersionca