Phospho-mimicking Atf1 mutants bypass the transcription activating function of the MAP kinase Sty1 of fission yeast

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• dc.contributor.author Sánchez Mir, Lauraca
• dc.contributor.author Salat Canela, Clàudiaca
• dc.contributor.author Paulo Mirasol, Esther, 1984-ca
• dc.contributor.author Carmona, Mercèca
• dc.contributor.author Ayté del Olmo, Joséca
• dc.contributor.author Oliva Miguel, Baldomeroca
• dc.contributor.author Hidalgo Hernando, Elenaca
• dc.date.accessioned 2018-01-25T11:36:15Z
• dc.date.issued 2017
• dc.description.abstract Stress-dependent activation of signaling cascades is often mediated by phosphorylation events, but the exact nature and role of these phosphorelays are frequently poorly understood. Here, we review which are the consequences of the stress-dependent phosphorylation of a transcription factor on gene activation. In fission yeast, the MAP kinase Sty1 is activated upon several environmental hazards and promotes cell adaptation and survival, greatly through activation of a gene program mediated by the transcription factor Atf1. Although described decades ago, the role of the phosphorylation of Atf1 by Sty1 is still a matter of debate. We present here a brief review of recent data, obtained through the characterization of several phosphorylation mutant derivatives of Atf1, demonstrating that Atf1 phosphorylation does not stabilize the factor nor stimulates its binding to DNA. Rather, it provides a structural platform of interaction with the transcriptional machinery. Based on these findings, future work will establish how this phosphorylated trans-activation domain promotes the massive gene expression shift allowing cellular adaptation to stress.
• dc.description.sponsorship This work was supported by the Ministerio de Economía y Competitividad (Spain), PLAN E and FEDER (BFU2015-68350-P to EH), and by 2014-SGR-154 from Generalitat de Catalunya (Spain) to EH. LSM is recipient of a Juan de la Cierva post-doctoral contract, and CSC is recipient of a María de Maeztu pre-doctoral fellowship, both from the Ministerio de Economía y Competitividad (Spain). EH is recipient of an ICREA Academia Award (Generalitat de Catalunya, Spain).
• dc.format.mimetype application/pdf
• dc.identifier.citation Sánchez-Mir L, Salat-Canela C, Paulo E, Carmona M, Ayté J, Oliva B. Phospho-mimicking Atf1 mutants bypass the transcription activating function of the MAP kinase Sty1 of fission yeast. Curr Genet. 2018 Feb;64(1):97-102. DOI: 10.1007/s00294-017-0730-7
• dc.identifier.doi http://dx.doi.org/10.1007/s00294-017-0730-7
• dc.identifier.issn 0172-8083
• dc.identifier.uri http://hdl.handle.net/10230/33749
• dc.language.iso eng
• dc.publisher Springerca
• dc.relation.ispartof Current Genetics. 2018 Feb;64(1):97-102
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2015-68350-P
• dc.rights © Springer The final publication is available at Springer via http://dx.doi.org/10.1007/s00294-017-0730-7
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.subject.keyword Transcription regulation
• dc.subject.keyword Schizosaccharomyces pombe
• dc.subject.keyword Phosphorylation
• dc.subject.keyword Sty1
• dc.subject.keyword Atf1
• dc.subject.keyword Oxidative stress
• dc.title Phospho-mimicking Atf1 mutants bypass the transcription activating function of the MAP kinase Sty1 of fission yeastca
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/acceptedVersion