A novel method of microsatellite genotyping-by-sequencing using individual combinatorial barcoding.

Mostra el registre complet Registre parcial de l'ítem

  • dc.contributor.author Vartia, Sallaca
  • dc.contributor.author Villanueva Cañas, José Luis, 1984-ca
  • dc.contributor.author Finarelli, Johnca
  • dc.contributor.author Farrell, Edward D.ca
  • dc.contributor.author Collins, Patrick C.ca
  • dc.contributor.author Hughes, Graham M.ca
  • dc.contributor.author Carlsson, Jeanette E. L.ca
  • dc.contributor.author Gauthier, David T.ca
  • dc.contributor.author McGinnity, Philipca
  • dc.contributor.author Cross, Thomas F.ca
  • dc.contributor.author FitzGerald, Richard D.ca
  • dc.contributor.author Mirimin, Lucaca
  • dc.contributor.author Crispie, Fionaca
  • dc.contributor.author Cotter, Paul D.ca
  • dc.contributor.author Carlsson, Jensca
  • dc.date.accessioned 2016-04-22T10:33:47Z
  • dc.date.available 2016-04-22T10:33:47Z
  • dc.date.issued 2016
  • dc.description.abstract This study examines the potential of next-generation sequencing based 'genotyping-by-sequencing' (GBS) of microsatellite loci for rapid and cost-effective genotyping in large-scale population genetic studies. The recovery of individual genotypes from large sequence pools was achieved by PCR-incorporated combinatorial barcoding using universal primers. Three experimental conditions were employed to explore the possibility of using this approach with existing and novel multiplex marker panels and weighted amplicon mixture. The GBS approach was validated against microsatellite data generated by capillary electrophoresis. GBS allows access to the underlying nucleotide sequences that can reveal homoplasy, even in large datasets and facilitates cross laboratory transfer. GBS of microsatellites, using individual combinatorial barcoding, is potentially faster and cheaper than current microsatellite approaches and offers better and more data.ca
  • dc.description.sponsorship S.V. and R.F. acknowledge funding from the Sea Change Strategy with the support of the Marine Institute and the Marine Research Sub-programme of the National Development Plan 2007–2013, co-financed by the European Regional Development Fund (Grant-Aid Agreement no. PBA/AF/07/004, EIRCOD). J.L.V.-C. acknowledges funding from the Spanish Ministerio de Economía y Competitividad (FPI BES-2010–038494 and EEBB-I-13–06270). G.H. acknowledges funding by the Irish Research Council (IRC) Graduate Research Education Programme (GREP). P.M. and J.C. acknowledge funding from the Beaufort Marine Research Award in Fish Population Genetics funded by the Irish Government under the Sea Change Programme. P.C.C. acknowledges funding from Science Foundation Ireland (SFI 12/IP/1308). E.D.F. acknowledges funding from Irish Research Council (IRC) (Funding Agency Ref no./nGOIPD/2013/320).
  • dc.format.mimetype application/pdfca
  • dc.identifier.citation Vartia S, Villanueva-Cañas JL, Finarelli J, Farrell ED, Collins PC, Hughes GM et al. A novel method of microsatellite genotyping-by-sequencing using individual combinatorial barcoding. R Soc Open Sci. 2016 Jan 20;3(1):150565. DOI: 10.1098/rsos.150565ca
  • dc.identifier.doi http://dx.doi.org/10.1098/rsos.150565
  • dc.identifier.issn 2054-5703
  • dc.identifier.uri http://hdl.handle.net/10230/26163
  • dc.language.iso engca
  • dc.publisher Royal Society Publishingca
  • dc.relation.ispartof Royal Society Open Science. 2016 Jan 20;3(1):150565
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BES2010-038494
  • dc.rights © 2016 Vartia , Villanueva-Cañas JL, Finarelli J, Farrell ED, Collins PC, Hughes GM. et al. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.ca
  • dc.rights.accessRights info:eu-repo/semantics/openAccessca
  • dc.rights.uri http://creativecommons.org/licenses/by/4.0/ca
  • dc.subject.other Regulació genètica -- Informàticaca
  • dc.subject.other Genòmica -- Informàticaca
  • dc.title A novel method of microsatellite genotyping-by-sequencing using individual combinatorial barcoding.ca
  • dc.type info:eu-repo/semantics/articleca
  • dc.type.version info:eu-repo/semantics/publishedVersionca

Col·leccions

Articles (Hospital del Mar Research Institute)
Articles (Departament de Medicina i Ciències de la Vida)