A transient contractile seam promotes epithelial sealing and sequential assembly of body segments

Godeau, Amélie L.; Marin-Riera, Miquel; Trubuil, Elise; Rogalla, Svana; Bengoetxea, Guillermo; Backová, Lenka; Pujol, Thomas; Colombelli, Julien; Sharpe, James; Martin-Blanco, Enrique; Solon, Jérôme

A transient contractile seam promotes epithelial sealing and sequential assembly of body segments

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dc.contributor.author Godeau, Amélie L.
dc.contributor.author Marin-Riera, Miquel
dc.contributor.author Trubuil, Elise
dc.contributor.author Rogalla, Svana
dc.contributor.author Bengoetxea, Guillermo
dc.contributor.author Backová, Lenka
dc.contributor.author Pujol, Thomas
dc.contributor.author Colombelli, Julien
dc.contributor.author Sharpe, James
dc.contributor.author Martin-Blanco, Enrique
dc.contributor.author Solon, Jérôme
dc.date.accessioned 2025-09-05T06:24:49Z
dc.date.available 2025-09-05T06:24:49Z
dc.date.issued 2025
dc.description.abstract In embryos, epithelial sealing proceeds with progressive zipping eventually leading to a scar-free epithelium and ensuring the assembly of body segments in insects and neural tube in mammals. How zipping is mechanically controlled to promote tissue fusion on long distances, remains unclear. Combining physical modeling with genetic and mechanical perturbations, we reveal the existence of a transient contractile seam that generates forces to reduce the zipping angle by force balance, consequently promoting epidermal sealing during Drosophila embryogenesis. The seam is formed by the adhesion of two tissues, the epidermis and amnioserosa, and is stabilized by the tensions generated by the segment boundaries. Once a segment is zipped, the seam disassembles concurrently with the inactivation of the Jun kinase pathway. Thus, we show that epithelial sealing is promoted by a transient actomyosin contractile seam allowing sequential segment assembly.
dc.description.sponsorship The research leading to these results has received funding from the Spanish Ministry of Economy and Competitiveness, Plan Nacional, BFU2015-68754, the Spanish Ministry of Science and Innovation, Plan National, PID2019-109117GB-100, funded by MICIU/AEI/10.13039/501100011033 and PID2022-142779NB-100, funded by MICIU/AEI/10.13039/501100011033 and by ERDF, EU, “Centro de Excelencia Severo Ochoa” and to the EMBL partnership. S.R. acknowledges the support by a fellowship from International Human Frontier Science Program, LT0007/2022-L. E.T. acknowledges the support by a fellowship by the UPV/EHU, PIF20/193. L.B. acknowledges the support received from the IKUR Strategy of the Basque Government. This study is part of the PIBA_2023_1_0033 project, funded by the Basque Government under the grant PIBA - PUE 2022. We acknowledge the support of the CERCA Program/Generalitat de Catalunya. This work was supported in part by the Fundaciòn Biofisika Bizkaia and the Basque Excellent Research Centre (BERC) program of the Basque Government.
dc.format.mimetype application/pdf
dc.identifier.citation Godeau AL, Marin-Riera M, Trubuil E, Rogalla S, Bengoetxea G, Backová L, et al. A transient contractile seam promotes epithelial sealing and sequential assembly of body segments. Nat Commun. 2025 Apr 29;16(1):4010. DOI: 10.1038/s41467-025-58566-z
dc.identifier.doi http://dx.doi.org/10.1038/s41467-025-58566-z
dc.identifier.issn 2041-1723
dc.identifier.uri http://hdl.handle.net/10230/71116
dc.language.iso eng
dc.publisher Nature Research
dc.relation.ispartof Nat Commun. 2025 Apr 29;16(1):4010
dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2015-68754
dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PID2019-109117GB-100
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PE/PID2022-142779NB-100
dc.rights © The Author(s) 2025. Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.keyword Cytoskeleton
dc.subject.keyword Morphogenesis
dc.subject.keyword Multicellular systems
dc.title A transient contractile seam promotes epithelial sealing and sequential assembly of body segments
dc.type info:eu-repo/semantics/article
dc.type.version info:eu-repo/semantics/publishedVersion

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